白藜芦醇对心肌梗死后心衰大鼠心室重构的作用及可能机制  

Effect and possible mechanism of resveratrol on ventricular remodeling in heart failure rats after myocardial infarction

作　　者：刘芳[1] 王一钧 那荣妹[1] 白倩茹 LIU Fang;WANG Yi-jun;NA Rong-mei;BAI Qian-ru(Department of Circulation,Zhongshan Hospital,Dalian University,Dalian 116001,China)

机构地区：[1]大连大学附属中山医院循环三科,辽宁大连116001

出　　处：《解剖科学进展》2023年第4期355-358,共4页Progress of Anatomical Sciences

基　　金：大连市医学科学研究计划(19Z11011)。

摘　　要：目的探究白藜芦醇对心肌梗死后心衰大鼠心室重构的作用及可能机制。方法30只SD大鼠随机分为假手术组(Sham组)、模型组(Model组)和白藜芦醇组(Res组),每组10只。超声诊断仪检测心脏射血分数、短轴缩短率和左心室舒张末径;HE染色观察心肌病理学改变;荧光探针检测心肌组织ROS水平;ELISA检测心肌组织氧化应激因子水平;Western blot检测心肌组织MMP-9及ASC/caspase-1通路蛋白表达水平。结果白藜芦醇增加心衰大鼠心脏射血分数和短轴缩短率,降低左心室舒张末径,改善心肌病理损伤,降低心肌组织ROS和MDA水平,增加SOD水平,降低心肌组织MMP-9、ASC、caspase1p20和IL-1β蛋白表达水平。结论白藜芦醇通过抑制ASC/caspase-1通路改善心肌梗死所致心衰大鼠心脏功能。Objective To explore the effect and possible mechanism of resveratrol on ventricular remodeling in rats with heart failure after myocardial infarction.Methods Thirty SD rats were randomly divided into sham group(Sham group),model group(Model group)and resveratrol group(Res group),with 10 rats in each group.Cardiac ejection fraction,short axis shortened rate and left ventricular end diastolic diameter were detected by ultrasonic diagnostic instrument.Pathological changes of myocardium were observed by HE staining.ROS level of myocardium tissue was detected by fluorescence probe,and oxidative stress factor level of myocardium tissue was detected by ELISA.The expression of MMP-9 and ASC/caspase-1 pathway proteins in myocardium was detected by Western blot.Results Resveratrol increased cardiac ejection fraction and short axis shortened rate,decreased left ventricular end-diastolic diameter,improved myocardial pathological injury,decreased level of ROS and MDA,increased level of SOD,and decreased expression of MMP-9,ASC,caspase1p20 and IL-1βin cardiac failure rats.Conclusion Resveratrol improves cardiac function in rats with heart failure caused by myocardial infarction by inhibiting ASC/caspase-1 pathway.

关 键 词：白藜芦醇 活性氧 ASC/caspase-1通路 心肌梗死 心衰 

分 类 号：R542.22[医药卫生—心血管疾病]