利用55K芯片进行小麦生育期相关性状的QTL定位及效应分析  

Mapping and effect analysis of QTL for phenology traits in wheat using 55K chip technology

作　　者：温明星 肖进[2] 徐涛[2] 孙丽[2] 王宗宽[2] 王海燕 王秀娥[2] WEN Ming-Xing;XIAO Jin;XU Tao;SUN Li;WANG Zong-Kuan;WANG Hai-Yan;WANG Xiu-E(Zhenjiang Institute of Agricultural Science,Jurong 212400,Jiangsu,China;State Key Laboratory of Crop Genetics and Germplasm Enhancement/Nanjing Agricultural University,Nanjing 210095,Jiangsu,China)

机构地区：[1]镇江市农业科学院,江苏句容212400 [2]作物遗传与种质创新利用全国重点实验室/南京农业大学,江苏南京210095

出　　处：《作物学报》2023年第12期3188-3203,共16页Acta Agronomica Sinica

基　　金：作物遗传与种质创新国家重点实验室开放基金项目(ZW20202009)资助。

摘　　要：生育期是决定小麦品种适应性的重要农艺性状,探索其遗传机制及效应对于小麦品种的选育和推广具有重要意义。本研究以扬麦158和密穗小麦杂交构建的240个重组自交家系(recombinant inbred line,RIL)为材料,于2~4个环境下对该群体主要生育期性状进行鉴定。利用已构建的高密度遗传图谱,共检测到52个生育期相关QTL,分布在2A、2D、3D、4B、4D、5A、5B、5D、6A、6B、7A、7B和7D染色体上。QJS/BS/HS/FS/MS.nau-5D.2、QJS/BS/HS/FS/MS.nau-2D.1和QJS/BS/HS/FS/MS.nau-7B.1均在多年被重复检测到,分别解释4.56%~46.86%、1.32%~33.40%和2.37%~13.27%的表型变异。QBS/HS.nau-2A.3、QHS/FS.nau-5B.2、QFS.nau-2A.5、QBS.nau-6A.2、QJS.nau-4D.2、QJS.nau-6A.3、QBS.nau-2A.2、QBS/HS.nau-6A.1、QFS.nau-7A.2、QMS.nau-3D、QMS.nau-4D.1和QMS.nau-6B.1是新发现的位点。效应分析表明,生育期相关QTL的聚合能不同程度地缩短生育期,可应用于培育早熟高产小麦品种。Phenology is an important agronomic trait for common wheat,which has a great impact to explore its genetic mechanism and effect for wheat breeding and application.In this study,240 recombinant inbred lines(RILs)derived from Yangmai 158 and Hiller were used to identify the phenology traits in 2–4 environments.A total of 52 QTL were detected on chromosomes 2A,2D,3D,4B,4D,5A,5B,5D,6A,6B,7A,7B,and 7D by using the constructed high-density genetic map.QJS/BS/HS/FS/MS.nau-5D.2,QJS/BS/HS/FS/MS.nau-2D.1,and QJS/BS/HS/FS/MS.nau-7B.1 were detected for several years,which explained 4.56%–46.86%,1.32%–33.40%,and 2.37%–13.27%of phenotypic variation,respectively.QBS/HS.nau-2A.3,QHS/FS.nau-5B.2,QFS.nau-2A.5,QBS.nau-6A.2,QJS.nau-4D.2,QJS.nau-6A.3,QBS.nau-2A.2,QBS/HS.nau-6A.1,QFS.nau-7A.2,QMS.nau-3D,QMS.nau-4D.1,and QMS.nau-6B.1 were new QTL.Pyramiding of multiple phenology loci with large effects or repeated is an effective approach to shorten the growth period in different degrees,which could be used to cultivate early-maturing and high-yield wheat varieties.

关 键 词：小麦 生育期 QTL SNP标记 

分 类 号：S512.1[农业科学—作物学]