烟草细胞质雄性不育系K326 MADS-box和SUPERMAN基因的特征  

Characteristics of MADS-box and SUPERMAN genes in tobacco cytoplasmic male sterile line K326

作　　者：崔芳芳 孟林峰 刘苗苗 张建强[2] 王建革[2] 刘齐元[1] CUI Fang-Fang;MENG Lin-Feng;LIU Miao-Miao;ZHANG Jian-Qiang;WANG Jian-Ge;LIUQi-Yuan(Key Laboratory of Crop Physiology,Ecology and Genetic Breeding,Ministry of Education/College of Agronomy,Jiangxi Agricultural University,Nanchang 330045,Jiangxi,China;College of Forestry,Jiangxi Agricultural University,Nanchang 330045,Jiangxi,China)

机构地区：[1]江西农业大学农学院/作物生理生态与遗传育种教育部重点实验室,江西南昌330045 [2]江西农业大学林学院,江西南昌330045

出　　处：《作物学报》2023年第12期3204-3214,共11页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(3196150292)资助。

摘　　要：细胞质雄性不育是杂种生产的重要工具,也是研究细胞核与细胞质互作的良好系统,但其机制仍不清楚。本研究以细胞质雄性不育系K326为材料,研究了烟草细胞质不育系雄蕊异常与花发育基因表达的关系。细胞质雄性不育系K326源于普通烟草天然不育株,用烤烟品种K326连续回交培育而成,不仅具有心皮化雄蕊现象,同时也有花瓣化雄蕊现象,异常雄蕊基部融合为一体。本研究首先用生信手段对控制普通烟草花发育的MADS-box基因和边界基因SUPERMAN进行了全基因组鉴定,分析了它们染色体定位和共线性,预测了B类基因和SUPEMAN基因的顺式作用元件,并研究了它们在不育系和保持系不同时期花蕾中的表达特征。结果表明,烟草共鉴定出160个MADS-box基因和5个SUPERMAN基因。这些MADS-box基因中有7个B类基因(4个PI基因,3个AP3基因),79个MADS-box基因定位于22条染色体上,3个SUPERMAN基因定位于3条染色体上。共线性分析表明,串联和片段DNA重复、倍加作用是MADS-box基因家族扩张的驱动力。观察发现,细胞质雄性不育系K326异常雄蕊在小蕾期就已出现,暗示细胞质雄性不育K326中雄蕊异常是早期分生组织发育缺陷的结果。qPCR检测表明,细胞质雄性不育系及保持系中可以检测到7个B类基因和1个SUPERMAN基因表达。PI基因表达水平NitMADS115在保持系各个时期均高于不育系;AP3基因NitMADS72、NitMADS100表达水平在保持系各个时期均低于不育系;其他基因呈现小蕾期和大蕾期下调,中蕾期上调。SUPERMAN基因只在保持系小蕾和中蕾期可以检测到,而不育系各个时期中均无法检测到;顺式作用元件分析表明,NitMADS115具有生长素响应元件AuxRR。因此,生长素可能在烟草细胞质逆行调控细胞核起重要作用。Cytoplasmic male sterility(CMS)is an important tool for hybrid production and a good system for studying the interaction between nucleus and cytoplasm,but its mechanism is still unclear.The relationship between stamen abnormality and the relative expression levels of flower development genes in tobacco CMS line K326 was studied.The CMS line K326 was derived from a natural variation in Nicotiana tabacum and was bred by continuous backcross of flue-cured tobacco variety K326.It has not only stamens with carpelloid,but also stamens with petaloid,and the base of abnormal stamens is fused into a whole.In this study,we identified MADS-box genes and SUPERMAN genes of whole genome,which controlled the flower development in N.tabacum and analyzed their chromosomal localization and collinearity.The cis-acting elements of B genes and SUPERMAN genes were predicted,and their expression characteristics in flower buds at different stages in both CMS line and its maintainer line were studied.The results showed that 160 MADS-box genes and 5 SUPERMAN genes were identified in the genome of tobacco,including 4 PI genes,3 AP3 genes,and 7 B genes.There were 79 MADS-box genes scattered on 22 chromosomes,and 3 SUPERMAN genes distributed on 3 chromosomes.Collinearity analysis showed that tandem and fragment DNA duplication and multiplication were the driving forces of the expansion of MADS-box gene family in tobacco.The abnormal stamens of CMS line K326 appeared at small bud stage,suggesting that the abnormal stamens in CMS line K326 were the result of early meristem development defects.The qRT-PCR showed that 7 B genes and 1 SUPERMAN gene were expressed both in CMS line K326 and its maintainers.The relative expression level of PI gene NitMADS115 in the maintainer line was higher than that in CMS line at all stages.The relative expression levels of AP3 genes NitMADS72 and NitMADS100 in maintainer lines were lower than those in CMS lines.Other genes were down-regulated at small bud stage and large bud stage,and up-regulated at middle bud

关 键 词：烟草 细胞质雄性不育 MADS-BOX SUPERMAN 生长素 

分 类 号：S572[农业科学—烟草工业]