基于高密度遗传图谱的水稻穗长QTL定位与分析  

Detection and analysis of QTL for panicle length in rice using a high-density genetic map

作　　者：韦敏益 张月雄[1] 马增凤[1] 黄大辉[1] 刘驰[1] 秦媛媛[2] 卢颖萍 鄢柳慧 吴子帅 周小龙 吴旭祥 秦钢[1] WEI Minyi;ZHANG Yuexiong;MA Zengfeng;HUANG Dahui;LIU Chi;QIN Yuanyuan;LU Yingping;YAN Liuhui;WU Zishuai;ZHOU Xiaolong;WU Xuxiang;QIN Gang(Rice Research Institute,Guangxi Academy of Agricultural Sciences/Guangxi Key Laboratory of Rice Genetics and Breeding,Nanning 530007,China;Agricultural Science and Technology Information Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,China;Liuzhou Branch,Guangxi Academy of Agricultural Sciences/Liuzhou Research Center of Agricultural Sciences,Liuzhou 545000,China;Guangdong Maoming Agriculture&Forestry Technical College,Maoming 525000,China)

机构地区：[1]广西农业科学院水稻研究所/广西水稻遗传育种重点实验室,广西南宁530007 [2]广西农业科学院农业科技信息研究所,广西南宁530007 [3]广西农业科学院柳州分院/柳州市农业科学研究中心,广西柳州545000 [4]广东茂名农林科技职业学院,广东茂名525000

出　　处：《华南农业大学学报》2023年第6期889-895,共7页Journal of South China Agricultural University

基　　金：广西科技计划(桂科AB21220016);广西自然科学基金(2021GXNSFDA075013,2022GXNSFAA035266);广西农业科学院基本科研业务专项及科技发展基金(桂农科2022JM21,桂农科2021JM05,桂农科2021YM04,桂农科2021YT027)。

摘　　要：【目的】深入挖掘与穗长相关的新基因,为水稻穗长调控的遗传机理研究及分子育种提供依据。【方法】以2个优良亲本‘ZP37’和‘R8605’及其杂交衍生的208个高世代重组自交系(Recombinant inbred lines,RILs)为作图群体,利用全基因组重测序高密度连锁图谱对3个不同环境下的穗长数量性状座位(Quantitative trait locus,QTL)进行定位,同时分析它们的聚合效应。【结果】共检测到11个穗长QTL,分别分布在第3、4、7、8、9和12号染色体上,其似然函数比对数值(Log of odds,LOD)介于3.07~12.87之间,贡献率在2.17%~10.94%之间,有7个QTL是新位点,其余4个QTL位点与已报道的穗长基因和QTL位置重叠或相近。在2个不同环境下重复检测到4个稳定的QTL位点;对聚合了不同数量穗长QTL株系的分析结果表明,穗长QTL表现出累加效应,QTL数量的增加能显著增加水稻穗长。【结论】本研究结果为水稻穗长QTL的克隆和功能解析奠定坚实的基础,为水稻高产育种提供理论依据和遗传资源。【Objective】To deeply explore new genes related to panicle length and provide a basis for the study of genetic mechanism of panicle length regulation and molecular breeding in rice.【Method】Two superior parents,‘ZP37’and‘R8605’,as well as 208 recombinant inbred lines(RILs)derived from the cross of ZP37/R8605 were used as a mapping population to locate quantitative trait loci(QTLs)for panicle length in three different environments through the high-density linkage map of whole genome resequencing,and to analyze their pyramiding effects.【Result】A total of 11 QTLs for panicle length were detected on chromosomes 3,4,7,8,9 and 12,with the logs of odds(LODs)ranging from 3.07 to 12.87 and contribution rates ranging from 2.17%to 10.94%,seven of the QTLs were new loci,and the remaining four QTLs overlapped or were close to the reported panicle length genes and QTLs.Among them,four stable QTLs were detected repeatedly in two different environments,and by analyzing the lines that pyramiding different numbers of panicle length QTLs,the results showed that the panicle length QTLs showed an additive effect,and the increase in the number of QTLs significantly increased the panicle length of rice.【Conclusion】The results of this study provide a solid foundation for cloning and functional analysis of rice panicle length QTLs,as well as a theoretical basis and genetic resources for high-yield rice breeding.

关 键 词：水稻 穗长 QTL定位 高密度遗传图谱 

分 类 号：S511[农业科学—作物学] S33