4个籼稻品种苗期耐热性评价及分子机理分析  

Evaluation of heat stress resistance and its molecular machanism of four indica rice cultivars at seedling stage

作　　者：叶婵娟 陈可 周新桥[1] 陈达刚[1] 郭洁 刘娟 曹哲 刘传光[1] 陈友订[1] 陈国荣[1] YE Chanjuan;CHEN Ke;ZHOU Xinqiao;CHEN Dagang;GUO Jie;LIU Juan;CAO Zhe;LIU Chuanguang;CHEN Youding;CHEN Guorong(Rice Research Institute,Guangdong Academy of Agricultural Sciences/Key Laboratory of Genetics and Breeding of High Quality Rice in Southern China(Co-construction by Ministry and Province),Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of New Technology in Rice Breeding/Guangdong Rice Engineering Laboratory,Guangzhou 510640,China;College of Life Sciences,South China Agricultural University,Guangzhou 510642,China)

机构地区：[1]广东省农业科学院水稻研究所/农业农村部华南优质稻遗传育种实验室(部省共建)/广东省水稻育种新技术重点实验室/广东省水稻工程实验室,广东广州510640 [2]华南农业大学生命科学学院,广东广州510642

出　　处：《华南农业大学学报》2023年第6期906-914,共9页Journal of South China Agricultural University

基　　金：广东省自然科学基金面上项目(2022A1515010796);肇庆市科技计划(2022N005);广东省重点领域研发计划(2022B0202060002);广东省驻镇帮镇扶村农村科技特派员团队项目(KTP20210405);国家自然科学基金(32101675);广东省重点实验室运行资金(2020B1212060047)。

摘　　要：【目的】明确4种不同籼稻品种苗期耐热性差异,为水稻品种选育与推广应用提供理论参考和技术支持。【方法】采用水稻基因组重测序、表型鉴定结合转录水平分析综合评价华南地区高产常规水稻品种‘南秀美占’、杂交稻恢复系‘R5518’及香稻品种‘九里香’和‘南晶香占’的苗期耐热性。【结果】‘南晶香占’苗期耐热性相对较弱,‘R5518’耐热性中等,‘九里香’和‘南秀美占’表现出苗期耐热性强于另外2个品种;通过比较水稻抗热相关基因在这4个品种的单倍型、荧光定量数据与表型数据,发现‘九里香’在OsTT1表现出较多的变异,而其他水稻耐热QTLs上,4个品种单倍型较为一致,可能OsTT1贡献了‘九里香’部分耐热性;表达模式上,OsHSF7、OsHSP71.1和OsHTS1基因表达趋势与水稻耐热性评价比较一致,表明这3个基因可能参与该研究中的4个籼稻品种耐热性调控。【结论】由于一些耐热相关基因的表达差异、基因移码、剪切转录本出错,造成基因失活,导致不同籼稻品种的苗期耐热性差异。该项研究结果可为水稻耐热性育种全基因组选择提供新的思路。【Objective】To identify the variation in heat stress resistance among seedlings of four different indica rice cultivars grown in South China,and provide theoretical reference and technical support for the breeding and promotion of rice varieties.【Method】We performed whole-genome re-sequencing,phenotype identification,and transcriptional level analysis to comprehensively evaluate the heat stress resistance forseedlings of high-yield conventional rice cultivar‘Nan Xiu Mei Zhan’(NXMZ),hybrid rice restorer cultivar‘R5518’,and aromatic rice cultivars‘Jiu Li Xiang’(JLX)and‘Nan Jing Xiang Zhan’(NJXZ)in South China.【Result】The‘NJXZ’was sensitive to heat stress.The‘R5518’showed medium resistance to heat stress.The resistances to heat stress of‘JLX’and‘NXMZ’at seedling stage were relatively high in comparison to other two rice cultivars.We compared the haplotypes of heat resistance related genes,the relative expression levels and phenotypes,and found that many SNPs appeared in OsTT1 from‘JLX’,while the haplotypes of four cultivars with other heat-related QTLs remained relatively consistent,suggesting that the OsTT1 might contribute to partial heat stress resistance in‘JLX’.The gene expression patterns in OsHSF7,OsHSP71.1 and OsHTS1 were consistent with the evaluation in heat stress resistance of rice cultivars,indicating that these three genes might associate with regulation in heat stress resistance of four indica rice cultivars.【Conclusion】The gene expression difference,gene shift and transcript error in certain genes result in variations in heat stress resistance of different indica rice cultivars at seedling stage.These results can provide new ideas for genome-wide selection for heat tolerance breeding in rice.

关 键 词：水稻品种 耐热性 苗期 重测序 分子机理 基因组变异 

分 类 号：S511[农业科学—作物学]