禽腺病毒血清4型中国流行株fiber2蛋白单克隆抗体制备与鉴定  

Preparation and identification of monoclonal antibody against fiber2 protein of a fowl adenovirus serotype 4 Chinese epidemic strain

作　　者：田开月 曲信芹 韩城昊 尹坤 杨盼盼 黄玉波 赵军[3] TIAN Kaiyue;QU Xinqin;HAN Chenghao;YIN Kun;YANG Panpan;HUANG Yubo;ZHAO Jun(Zhoukou Academy of Agricultural Sciences,Zhoukou 466000,China;Qingdao Vland Biotech INC,Qingdao 266000,China;College of Veterinary Medicine,Henan Agricultural University,Zhengzhou 450046,China)

机构地区：[1]周口市农业科学院,河南周口466000 [2]青岛蔚蓝生物股份有限公司,山东青岛266000 [3]河南农业大学动物医学院,河南郑州450046

出　　处：《畜牧与兽医》2023年第11期104-109,共6页Animal Husbandry & Veterinary Medicine

基　　金：国家重点研发计划项目(2016YFD0500800)。

摘　　要：旨在制备禽腺病毒血清4型(fowl adenovirus serotype 4,FAdV-4)中国流行株的fiber2蛋白的特异性单克隆抗体。以纯化的FAdV-4中国流行株CH/HNJZ/2015免疫BALB/c小鼠,取小鼠脾脏与SP2/0细胞融合,采用基于fiber2蛋白的间接ELISA筛选阳性杂交瘤细胞,经过5次亚克隆,获得2株分泌抗fiber2蛋白抗体的杂交瘤细胞株,分别命名为2C3和7B4。以制备的2C3和7B4腹水进行亚型鉴定、反应原性、特异性以及中和活性测定。结果显示,成功制备了抗FAdV-4 fiber2蛋白的特异性单克隆抗体,获得的单克隆抗体均为IgM亚型,可特异性识别FAdV-4及其fiber2蛋白,为FAdV-4的免疫检测和fiber2蛋白功能研究提供了研究材料。This study was to prepare a specific monoclonal antibody(mAb)against fiber2 protein of a fowl adenovirus serotype 4(FAdV-4)Chinese epidemic strain.The purified FAdV-4 strain CH/HNJZ/2015 was used as an immunogen to immunize BALB/c mice.The spleen lymphocytes from the immunized mice were fused with SP2/0 cells.Positive hybridoma clones were screened by a fiber2-based indirect ELISA.Two hybridoma cell clones secreting anti-fiber2 antibodies,2C3 and 7B4 were obtained after five rounds of subcloning.The subtype,reactivity,specificity,and neutralizing activity of the prepared 2C3 and 7B4 ascites were identified.The results showed that specific mAbs a⁃gainst FAdV-4 fiber2 protein was successfully prepared.The subtype of mAbs was IgM.The mAbs was able to specifically recognize FAdV-4 and fiber2 protein,which would serve as materials for research on immune detection of FAdV-4 and for function study of fiber2.

关 键 词：禽腺病毒血清4型 fiber2蛋白 单克隆抗体 

分 类 号：S852[农业科学—基础兽医学]