基于BSA分析技术对陆地棉抗旱基因的鉴定  

QTL mapping and genomic selection of maize leaf width

作　　者：孙丰磊 任姣姣 雷斌[2] 高文伟[1] 曲延英[1] SUN Fenglei;REN Jiaojiao;LEI Bin;GAO Wenwei;QU Yanying(College of Agronomy,Xinjiang Agricultural University,Urumqi 830052,China;Institute of Nuclear Technology and Biotechnology,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China)

机构地区：[1]新疆农业大学农学院,乌鲁木齐830052 [2]新疆农业科学院核技术生物研究所,乌鲁木齐830052

出　　处：《新疆农业科学》2023年第10期2341-2351,共11页Xinjiang Agricultural Sciences

基　　金：新疆维吾尔自治区自然科学基金项目“棉花抗旱表型鉴定及基因定位”(2019D01A41);博士后面上项目“棉花关键抗旱表型鉴定及基因定位”(2018M64377)。

摘　　要：【目的】研究抗旱相关的候选基因,为棉花抗旱性鉴定与评价和抗旱基因的挖掘提供理论依据。【方法】以陆地棉石远321和奎85-174为亲本构建重组自交系中选取的20份极端抗旱和旱敏感材料,将筛选的极端材料分别构建极端抗旱池、极端敏旱池,与亲本进行BSA测序分析,以陆地棉基因组为参考,通过BSA测序分析,挖掘抗旱相关的候选基因。【结果】关联到3个相关的候选区域,候选区域的总长度为6.12 Mb,其中共包含86个基因。结合GO注释、KEGG通路和前期筛选出的5个关键指标,共筛选出5个候选基因。【结论】5个基因在叶片中的表达量显著高于根和茎,并且在抗旱性强材料中的表达量均高于抗旱性弱的材料,且总体呈先上升后下降的趋势。【Objective】To discover candidate genes related to drought resistance in the hope of providing theoretical basis for subsequent identification and identification and evaluation of drought resistance of cotton and research on drought resistance genes.【Methods】In this experiment,20 extreme drought and drought sensitive materials selected from recombinant inbred lines were constructed from Upland Cotton Shiyuan 321 and Kui 85-174.The extreme drought and extremely sensitive drought pools were constructed respectively,BSA sequencing was analyzed with the parents,and candidate genes were mined by BSA sequencing analysis using the upland cotton genome as a reference.【Results】The results showed that they were finally associated with 3 related candidate regions.The total length of the candidate regions was 6.12 Mb,which contained a total of 86 genes.Combining GO annotation,KEGG pathway and five key indicators selected in the previous stage,through qRT-PCR detection and analysis,a total of 5 candidate genes were finally determined.【Conclusion】The expression levels of the five genes in leaves were significantly higher than those in roots and stems.And the expression levels in drought-resistant materials were all higher materials with weak drought resistance,and the overall trend was“rising first and then falling”.

关 键 词：陆地棉 抗旱性 BSA 基因表达 

分 类 号：S562[农业科学—作物学]