42份匍匐型花生幼苗对盐胁迫的生理响应  

Physiological response of 42 creeping peanut cultivars to salt stress at seedling stage

作　　者：姚庆 施俊杰 侯献飞 贾东海[2] 顾元国[2] 阿里别里根·哈孜太 苗昊翠[3] 李强[2] YAO Qing;SHI Junjie;HOU Xianfei;JIA Donghai;GU Yuanguo;Aribelegan Hazzetti;MIAO Haocui;LI Qiang(Institute of Agricultural Sciences of Ili Prefecture,Ining Xinjiang 835000,China;Institute of Economic Crops,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China;Institute of Crop Variety Resources,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China)

机构地区：[1]新疆伊犁哈萨克自治州农业科学研究所,新疆伊宁835000 [2]新疆农业科学院经济作物研究所,乌鲁木齐830091 [3]新疆农业科学院农作物品种资源研究所,乌鲁木齐830091

出　　处：《新疆农业科学》2023年第10期2442-2452,共11页Xinjiang Agricultural Sciences

基　　金：新疆维吾尔自治区重大科技专项“油料作物种质资源收集与优异基因挖掘关键技术研究”(2022A03004-4);新疆维吾尔自治区天山英才青年拔尖人才项目(2021-2023年);国家花生产业技术体系“新疆综合试验站”(CARS-13)。

摘　　要：【目的】研究42份匍匐型花生品种在盐胁迫下的生理变化,为筛选耐盐花生品种提供依据。【方法】利用水培法,在200 mmol/L NaCl胁迫浓度下对参试花生材料幼苗进行胁迫,测定叶片的脯氨酸(Pro)、丙二醛(MDA)、叶绿素(Chl)、可溶性糖4个生理指标,运用隶属函数法进行综合分析。【结果】(1)幼苗期花生叶片脯氨酸(Pro)含量全部呈上升趋势;(2)丙二醛(MDA)含量除C095、Z5036、Z5081、C094、C108、C128、C141、C145、C145-2、Z5096、Z5108、Z5123、Z5157、Z5185、Z5230、C108-116个品种上升外,大多呈现下降趋势,表现出较好的耐盐响应;(3)80%的花生品种叶绿素(Chl)含量较CK上升,而C905、Z5036、Z5040、C035、C087、C129、C135、Z5083、Z52259个品种叶绿素含量降低;(4)可溶性糖含量大部分呈现上升趋势,只有Z5035、C001、C087、C128、C135、C141、C145、C145-1、C145-2、Z5028、Z5123、Z5157、Z522513个品种叶绿素含量下降,属于盐敏感品种。【结论】42个匍匐型花生品种分为4类耐盐群体,分别为高耐盐群体、耐盐群体、中等耐盐群体、盐敏感群体。【Objective】To study the physiological changes of 42 creeping peanut varieties under salt stress.【Methods】Hydroponic method was used to measure the physiological indexes of proline(Pro),malondialdehyde(MDA),chlorophyll(Chl)and soluble sugar in the leaves of seedlings under the short-term stress concentration of 200 mmol/LNaCl.The comprehensive analysis was carried out by membership function method in order to provide a basis for screening salt-tolerant peanut varieties.【Results】(1)The proline(Pro)content of peanut leaves increased in seedling stage;(2)The content of malondialdehyde(MDA)increased except for 16 varieties c095,Z5036,Z5081,C094,C108,C128,C141,C145,C145-2,Z5096,Z5108,Z5123,Z5157,Z5185,Z5230,C108-1,and other varieties showed a downward trend and good salt tolerance response.(3)The chlorophyll(Chl)content of 80%peanut varieties increased compared with CK,while the contents of C905,Z5036,Z5040,C035,C087,C129,C135,Z5083,Z5225 decreased.(4)Compared with CK,most of the soluble sugar content showed an upward trend,and only the chlorophyll content of Z5035,C001,C087,C128,C135,C141,C145,C145-1,C145-2,Z5028,Z5123,Z5157,Z5225 decreased,which belonged to salt sensitive varieties.【Conclusion】Cluster analysis is used to classify 42 creeping peanut varieties into four salt tolerance groups,namely,high salt tolerance group,salt tolerance group,medium salt tolerance group and salt sensitive group.

关 键 词：匍匐型花生 NACL胁迫 生理指标 综合抗盐能力 

分 类 号：S663.1[农业科学—果树学]