外源褪黑素对NaCl胁迫下草莓幼苗生理特性的缓解效应  

Alleviating effect of exogenous melatonin on strawberry seedling under NaCl stress

作　　者：马媛媛 李刚[2] 何旺 赵宝龙[1] 鲁倩君 陈丽靓 刘迎 MA Yuanyuan;LI Gang;HE Wang;ZHAO Baolong;LU Qianjun;CHEN Liliang;LIU Ying(College of Agronomy,Shihezi University/Key Laboratory of Physidogy and Germplasm Resource Utili zation of Special Fruit and Vegetable Cultivation in the Corps,Shihezi Xinjiang 832000,China;Zhengzhou Fruit Tree Research Institute,Chinese Academy of Agricultural Sciences,Zhengzhou 450009,China;Chongqing Qionjang District Agricultural Technology Service Center,Chongqing 400000,China)

机构地区：[1]石河子大学农学院/特色果蔬栽培生理与种质资源利用兵团重点实验室,新疆石河子832000 [2]中国农业科学院郑州果树研究所,郑州450009 [3]重庆市黔江区农业技术服务中心,重庆400000

出　　处：《新疆农业科学》2023年第10期2486-2495,共10页Xinjiang Agricultural Sciences

基　　金：新疆果树类品种筛选及技术示范推广(Y2021XK14)。

摘　　要：【目的】研究NaCl胁迫下不同浓度褪黑素对草莓幼苗生长指标、光合色素含量、光合特性、叶绿素荧光特性和抗氧化酶活性的影响,为分析褪黑素缓解草莓NaCl胁迫的最适浓度提供理论依据。【方法】以甜查理为材料,测定幼苗在5 g/L NaCl处理下,外源施加不同浓度褪黑素(0、50、100、150和200μmol/L),测定第3、6和9 d的生长指标、光合色素含量、光合特性、叶绿素荧光和抗氧化酶活性。【结果】NaCl胁迫下草莓幼苗的生长指标、光合色素含量、光合特性、叶绿素荧光特性和抗氧化酶活性显著降低。外源施加50、100、150和200μmol/L浓度的褪黑素可不同程度的增加NaCl胁迫下草莓幼苗叶片生长指标、光合色素含量、光合特性、叶绿素荧光特性和抗氧化酶活性。褪黑素150μmol/L的浓度效果为最佳,处理第9 d,与NaCl胁迫相比,草莓幼苗叶片的株高、茎粗、单株重、Chla、Chlb、Car、Pn、Ci、Fm、SOD、CAT、POD和APX分别增长了23.75%、37.97%、18.87%、37.95%、75.93%、41.22%、58.96%、26.47%、46.72%、32.89%、84.26%、48.34%和33.94%,F _(0)的上升速度显著降低。【结论】在NaCl胁迫下,外源施加不同浓度的褪黑素均可有效缓解NaCl胁迫对草莓造成的伤害,其中150μmol/L效果最佳。【Objective】To study NaCl stress,different concentrations of exogenous melatonin on growth indicators,photosynthetic pigment content,photosynthetic characteristics,chlorophyll fluorescence characteristics and antioxidant activity of strawberry seedlings in the hope of providing the optimal concentration of stress of strawberry NaCl stress for melatonin theoretical basis.【Methods】Strawberry seedlings were taken as testing materials,and the seedlings were measured under 5 g/L NaCl treatment by spraying different concentrations of exogenous melatonin(0,50,100,150 and 200μmol/L).During the experiments,growth indicators,photosynthetic content,photosynthetic properties,chlorophyll fluorescence and antioxidant activity were determined the 3,6 and 9 d.【Results】NaCl stress could reduce the growth index,photosynthetic properties,photosynthetic properties,chlorophyll fluorescence characteristics,and antioxidant activity of strawberry seedlings.Exogenous application of 50,100,150 and 200μmol/L concentrations of melatonin increased them under NaCl stress.The concentration effect of melatonin 150μmol/L was optimal,and after being processed 9 d,compared with NaCl stress,the plant height,stem diameter,fresh weight per plant,Chla,Chlb,Car,Pn,Ci,Fm,SOD,CAT,POD and APX of strawberry seedlings increased by 23.75%,37.97%,18.87%,37.95%,75.93%,41.22%,58.96%,26.47%,46.72%,32.89%,84.26%,48.34%and 33.94%,F _(0) rising speed was significantly reduced.【Conclusion】Under NaCl stress,spraying different concentrations of melatonin can effectively alleviate NaCl stress injury to strawberries and the best effects is 150μmol/L.

关 键 词：草莓 褪黑素 NACL胁迫 光合特性 

分 类 号：S668.4[农业科学—果树学]