也迷里鸡微卫星遗传多样性分析  

Analysis of genetic diversity of Yemili chicken by microsatellite

作　　者：姚莹莹 李家辉 李海英[1] 吴盈萍 赵晓钰[1] 周军 赵全庄 李宗福 YAO Yingying;LI Jiahui;LI Haiying;WU Yingping;ZHAO Xiaoyu;ZHOU Jun;ZHAO Quanzhuang;LI Zongfu(College of Animal Sciences,Xinjiang Agricultural University,Urumqi 830052,China;Animal Husbandry Science and Technology Research and Extension Center of Tacheng Prefecture,Tacheng Xinjiang 834700,China;Emin County National Flying Protection and Breeding Field,Emin Xinjiang 834606,China)

机构地区：[1]新疆农业大学动物科学学院,乌鲁木齐830052 [2]塔城地区畜牧科技研究推广中心,新疆塔城834700 [3]新疆额敏县国家级飞鹅保种场,新疆额敏834606

出　　处：《新疆农业科学》2023年第10期2574-2582,共9页Xinjiang Agricultural Sciences

基　　金：新疆维吾尔自治区畜禽种业提升项目(2022xjjq-z-01);新疆维吾尔自治区创新环境(重点实验室L)(2019D04011)。

摘　　要：【目的】研究也迷里鸡遗传多样性变化及世代间遗传分化,为也迷里鸡品种选育提供理论依据。【方法】利用15个微卫星位点对也迷里鸡2个世代共4个群体(G_(1):1世代公鸡、G_(2):2世代公鸡、M _(1):1世代母鸡、M _(2):2世代母鸡)进行遗传多样性分析。【结果】15个微卫星位点在120个个体中共检测到102个等位基因,每个位点平均等位基因数(Na)为6.8个。各位点的期望杂合度(He)和观测杂合度(Ho)平均值分别为0.708和0.660。平均多态信息含量(PIC)较高(0.661),表现为高度多态性。F-统计量中群体内近交系数(Fis)、总群体近交系数(Fit)和群体间遗传分化系数(Fst)平均值分别为0.051、0.070和0.020,也迷里鸡群体的近交程度低、遗传分化不明显。群体基因流MCW0216位点最大(29.689),LEI0166位点最小(6.192),各位点平均基因流(Nm)为15.091,4个群体间存在高度的基因交流。也迷里鸡4个群体按遗传距离被分为2类。【结论】也迷里鸡具有较大的选择潜力,拥有丰富的遗传多样性。【Objective】The purpose of this study is to explore the genetic diversity and genetic differentiation of Yemili aboriginal chickens in the hope of providing theoretical basis for the breeding of this breed.【Methods】In this study,15 microsatellite loci were used to analyze the genetic diversity of four groups(G_(1):1 generatons,G_(2):2 generatons,M _(1):1 generatons,and M _(2):2 generatons).【Results】The results showed that a total of 102 alleles were detected in 15 microsatellite loci in 120 individuals,and the average number of alleles(Na)per locus was 6.8.The average expected heterozygosity(He)and observed heterozygosity(Ho)values for the points were 0.708 and 0.660,respectively.The average polymorphic information content(PIC)(0.661)was highly polymorphism.The mean intra-population inbreeding coefficient(Fis),total population inbreeding coefficient(Fit)and inter-population genetic differentiation coefficient(Fst)were 0.051,0.070 and 0.020,respectively,indicating that the low degree of inbreeding and genetic differentiation were not obvious.The population gene flow MCW0216 site was the largest(29.689),the smallest LEI0166 site(6.192),the average gene flow(Nm)was 15.091,and a high degree of gene communication among the four populations.Cluster analysis showed that four groups of Yemili aboriginal chickens were divided into two groups according to genetic distance.【Conclusion】In conclusion,Yemili aboriginal chickens have great selection potential and rich genetic diversity.

关 键 词：也迷里鸡 微卫星标记 遗传多样性 多态信息含量 遗传距离 

分 类 号：S831[农业科学—畜牧学]