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作 者:王彦钦 孟宪刚[1] 李武阳 罗光宏 WANG Yanqin;MENG Xiangang;LI Wuyang;LUO Guanghong(College of Biological and Pharmaceutical Engineering,Lanzhou Jiaotong University,Lanzhou 730070,China;Microalgae Engineering Research Center of Gansu Province,Hexi University,Zhangye 734000,China)
机构地区:[1]兰州交通大学生物与制药工程学院,甘肃兰州730070 [2]河西学院甘肃省微藻工程技术研究中心,甘肃张掖734000
出 处:《河南农业科学》2023年第10期22-29,共8页Journal of Henan Agricultural Sciences
基 金:甘肃省科技计划项目(18JR2JG001)。
摘 要:以正常生长的小麦幼苗为对照(CK),研究衣藻藻液[0 g/L(T0)、0.25 g/L(T1)、0.5 g/L(T2)、1.0 g/L(T3)和2.0 g/L(T4)]对盐胁迫下小麦幼苗叶片光合色素含量、光合气体交换参数以及叶绿素荧光参数等光合生理指标的影响,为衣藻在小麦盐胁迫下的应用提供理论依据。结果表明,CK小麦幼苗叶片光合色素含量、净光合速率(Pn)、气孔导度(Gs)、CO_(2)浓度(Ci)、蒸腾速率(Tr)、光系统Ⅱ实际光量子产量[Y(Ⅱ)]、最大光化学效率(Fv/Fm)、电子传递速率(ETR)、光化学淬灭系数(qP)均最高,非光化学淬灭系数(qN)最低。T1—T4处理小麦幼苗叶片叶绿素a、总叶绿素和类胡萝卜素含量均高于T0处理,以T3、T4处理较高。T1—T4处理Pn、Gs、Ci、Tr在处理后6~12 d均高于T0处理,总体以T3处理最高,T4处理次之。T1—T4处理Fv/Fm、ETR、Y(Ⅱ)、qP在处理后6~12 d总体上均高于T0处理,以T3处理最高,T4处理次之;T1—T4处理qN在处理后6~12 d均低于T0处理。综合考虑,1.0 g/L衣藻藻液效果最佳。The wheat seedlings under normal condition were as control(CK),the effects of Chlamydomonas algal liquid[0 g/L(T0),0.25 g/L(T1),0.5 g/L(T2),1.0 g/L(T3)and 2.0 g/L(T4)]on photosynthetic pigment content,photosynthetic gas exchange parameters and chlorophyll fluorescence parameters of wheat seedlings under high salt stress were investigated,so as to provide a theoretical basis for the application of Chlamydomonas algal liquid in wheat under salt stress.The results showed that wheat seedlings of CK had the highest photosynthetic pigment content,net photosynthetic rate(Pn),stomatal conductance(Gs),CO_(2) concentration(Ci),transpiration rate(Tr),actual optical quantum yield of optical systemⅡ[Y(Ⅱ)],maximum photochemical efficiency(Fv/Fm),electron transport rate(ETR),photochemical quenching coefficient(qP),and the lowest non‑photochemical quenching coefficient(qN).The contents of chlorophyll a,total chlorophyll and carotenoids of T1—T4 treatments were higher than those in T0 treatment,especially T3 and T4 treatments.The Pn,Gs,Ci and Tr of T1—T4 treatments were higher than those of T0 treatment at 6—12 days after treatment,and T3 treatment was generally the highest,followed by T4 treatment.The Fv/Fm,ETR,Y(Ⅱ)and qP of T1—T4 treatments were overall higher than those of T0 treatment at 6—12 days after treatment,and T3 treatment was generally the highest,followed by T4 treatment;qN of T1—T4 treatments were lower than that of T0 treatment at 6—12 days after treatment.In general,1.0 g/L Chlamydomonas algal liquid has the best effect.
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