柞树叶总黄酮对四氧嘧啶致糖尿病小鼠血糖的影响及其药效物质基础研究  

Study on hypoglycemic activity and pharmacodynamic material basis of Quercus mongolica leaves total flavonoids on alloxan-induced diabetic rats

作　　者：陈喜生 杜茂波[2] Chen Xisheng;Du Maobo(Institute of Life Sciences,Sinopharm Xingsha Pharmaceuticals(Xiamen)Co.,Ltd.,Xiamen 361028,China;Traditional Chinese Medicine Preparation Research Center,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]国药控股星鲨制药(厦门)有限公司生命科学研究院,厦门361028 [2]中国中医科学院中药研究所中药制剂研究中心,北京100700

出　　处：《国际中医中药杂志》2023年第10期1246-1252,共7页International Journal of Traditional Chinese Medicine

摘　　要：目的观察柞树叶总黄酮对四氧嘧啶诱发高血糖小鼠的降血糖作用,探讨其潜在机制及药效物质基础。方法将健康ICR小鼠按随机数字表法分为正常组和造模组,造模组小鼠尾静脉注射四氧嘧啶生理盐水溶液(45 mg/kg)建立化学性糖尿病模型。将造模组小鼠分为模型组,阳性药组(盐酸二甲双胍150 mg/kg),总黄酮低(100 mg/kg)、中(200 mg/kg)、高(400 mg/kg)剂量组,单体黄酮低(25 mg/kg)、中(50 mg/kg)、高(100 mg/kg)剂量组。各给药组均按0.2 ml/10 g小鼠体重灌胃给药,1次/d,连续灌胃14 d,正常组和模型组灌胃等体积生理盐水。分别于给药第7、14天,测定小鼠体重及空腹血糖值;计算血糖曲线下面积(AUC);测定血清胰岛素和胰岛素受体β浓度,计算胰岛素敏感指数;利用超高效液相色谱-串联质谱(UPLC-MS/MS)技术分析柞树叶总黄酮中的主要化学成分。结果与模型组比较,给药14 d,柞树叶总黄酮各剂量组及槲皮素3-O-β-D吡喃葡萄糖苷和紫云英苷各剂量组血糖值降低(P<0.01或P<0.05),AUC下降(P<0.01或P<0.05),胰岛素敏感指数升高(P<0.01或P<0.05);UPLC-MS/MS鉴定了柞树叶总黄酮中12个黄酮苷及苷元类成分。结论推测柞树叶降糖的物质基础为其黄酮类化合物,其中柞树叶总黄酮及槲皮素3-O-β-D吡喃葡萄糖苷和紫云英苷单体黄酮具有一定的降血糖活性,可通过改善胰岛素抵抗而发挥降血糖作用。Objective To study the hypoglycemic effect of Quercus mongolica leaves total flavonoids on alloxan-induced hyperglycemia mice;To discuss its underlying mechanism and pharmacodynamic material basis.Methods The healthy ICR mice were divided into control group and diabetic mice group according to random number table method.The diabetic mouse model was established by intravenously injected with streptozotocin solution(45 mg/kg)via the tail vein.The model diabetic mice were divided into model group,positive drug group(150 mg/kg metformin hydrochloride),total flavonoids low(100 mg/kg),medium(200 mg/kg)and high(400 mg/kg)dose groups,and monomer flavonoids low(25 mg/kg),medium(50 mg/kg)and high(100 mg/kg)dose groups.Each group of mice received 0.2 ml/10 g body weight for gavage,once a day for 14 consecutive days.The control and model group were given an equal volume of physiological saline.On the 7th and 14th day of administration,the body weight,fasting blood glucose,area under the curve(AUC)of blood glucose,serum insulin and insulin receptor beta concentrations,and insulin sensitivity index were measured.The main chemical components of total flavonoids from Quercus mongolica leaves were analyzed using ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)technology.Results Compared with the model group,after 14 days of administration,the total flavonoids of Quercus mongolica leaves at different doses and quercetin-3-O-β-D-glucopyranoside and astragalin at different doses all significantly reduced blood glucose levels(P<0.01 or P<0.05),decreased AUC(P<0.01 or P<0.05),and increased insulin sensitivity index(P<0.01 or P<0.05).The 12 flavone glycosides and aglycone components were identified by UPLC-MS/MS.Conclusions Flavonoids are speculated to be the material basis for the hypoglycemic effect of Quercus mongolica leaves.Among them,total flavonoids of Quercus mongolica leaves and monomeric flavonoids such as quercetin 3-O-β-D-glucopyranoside and astragalin have certain hypoglycemic activity,wh

关 键 词：糖尿病 柞树叶总黄酮 中药化学成分 串联质谱法 降血糖 小鼠 

分 类 号：R285.5[医药卫生—中药学]