不同配伍比例石膏-知母药对合煎与单煎化学成分差异性研究  

Contrastive analysis on difference of chemical compositions of single decoction and mixed decoction of Gypsum Fibrosum-Anemarrhenae Rhizoma medicinal pair with different proportion

作　　者：周颖欣 黄瑶 姚晓璇 田甜 林嘉明 孙建焱 张志鹏 孙冬梅 Zhou Yingxin;Huang Yao;Yao Xiaoxuan;Tian Tian;Lin Jiaming;Sun Jianyan;Zhang Zhipeng;Sun Dongmei(Guangdong Yi Fang Pharmaceutical Co.,Ltd.,Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula Granule,Foshan 528244,China)

机构地区：[1]广东一方制药有限公司广东省中药配方颗粒企业重点实验室,佛山528244

出　　处：《国际中医中药杂志》2023年第10期1281-1287,共7页International Journal of Traditional Chinese Medicine

基　　金：佛山市核心技术攻关项目(1920001000378);佛山市应急科技攻关专项(2020001000206)。

摘　　要：目的比较不同配伍比例石膏-知母药对合煎与单煎化学成分的差异性。方法采集不同配伍比例石膏-知母药对合煎与单煎样品的超高效液相色谱(UPLC)和离子色谱指纹图谱,运用SPSS 26.0软件对指纹图谱共有峰的“峰面积/取样量”比值进行独立样本t检验。结果不同配比(1∶1、3∶2、2∶1、5∶2、3∶1)石膏-知母药对合煎与单煎样品的UPLC指纹图谱中峰1、峰2、峰3、峰6、峰13的“峰面积/取样量”值比较,差异有统计学意义(P<0.05),而峰5、峰11的“峰面积/取样量”值比较差异无统计学意义(P>0.05);当配比为1∶1、3∶2、5∶2、3∶1时,峰7(新芒果苷)的“峰面积/取样量”值比较差异有统计学意义(P<0.05)。不同配比(1∶1、3∶2、2∶1、5∶2、3∶1)的离子色谱指纹图谱中,峰1的“峰面积/取样量”值比较,差异有统计学意义(P<0.05),而峰5(Ca2+)的“峰面积/取样量”值比较,差异无统计学意义(P>0.05)。结论不同配伍比例的石膏-知母药对合煎与单煎化学成分存在一定差异。Objective To compare the difference of chemical compositions of single decoction and mixed decoction of Gypsum Fibrosum-Anemarrhenae Rhizoma medicinal pair with different proportion.Methods The Ultra Performance Liquid Chromatography(UPLC)and Ion Chromatography(IC)fingerprints of each sample were collected,and the independent sample t-test on the ratio of"peak area/weight"value of common peaks which was from the single decoction and mixed decoction samples was performed through SPSS 26.0.Results In the UPLC fingerprint,there was significant difference in the"peak area/weight"values of peak 1,peak 2,peak 3,peak 6 and peak 13 between mixed decoction and single decoction with different compatibility ratios(1:1,3:2,2:1,5:2,3:1)(P<0.05),while peak 5 and peak 11 were without significant difference(P>0.05).When the compatibility ratio of Gypsum Fibrosum and Anemarrhenae Rhizoma was 1:1,3:2,5:2,3:1,there was significant difference in the"peak area/weight"values of peak 7(neomangiferin)(P<0.05).In the IC fingerprint,there was significant difference in the"peak area/weight"values of peak 1 between mixed decoction and single decoction with different compatibility ratios(1:1,3:2,2:1,5:2,3:1)(P<0.05),while there was no significant difference in peak 5(calcium ion)(P>0.05).Conclusion There are differences in the chemical component content of Gypsum Fibrosum-Anemarrhenae Rhizoma medicinal pair for single decoction and mixed decoction.

关 键 词：石膏 知母 药对 中药配伍 煎煮法 指纹图谱 

分 类 号：R289.1[医药卫生—方剂学]