小檗碱对电离辐射损伤大鼠的保护作用及机制  

Protective effect of berberine against ionizing radiation injury in rats and its mechanism of action

作　　者：陈吉刚 殷爱民 姚叶豹 王晓婷 蒋德娟 李清果 曹务锐 罗莹莹 刘承军 CHEN Jigang;YIN Aimin;YAO Yebao;WANG Xiaoting;JIANG Dejuan;LI Qingguo;CAO Wurui;LUO Yingying;LIU Chengjun(Hospital Laoshan Medical Sector,Qingdao 266101 China)

机构地区：[1]第971医院崂山医疗区,山东青岛266101

出　　处：《中国辐射卫生》2023年第4期474-478,共5页Chinese Journal of Radiological Health

基　　金：山东省医药卫生科技发展计划项目(2019WS607)。

摘　　要：目的探究小檗碱(BBR)对电离辐射损伤大鼠的保护作用及机制。方法把SD大鼠分为7组:正常对照组、1 Gy组、1 Gy低、高剂量BBR组(50,150 mg/kg)、3 Gy组、3 Gy低、高剂量BBR组(50,150 mg/kg)。除正常对照组外,各组大鼠进行医用电子直线加速器外照射,照后BBR灌胃给药,连续10 d。微量法测定大鼠血清超氧化物歧化酶(SOD)、还原型谷胱甘肽(GSH)和丙二醛(MDA)水平,HE染色法观察骨髓、小肠病理变化。结果与正常对照组比较,辐射组大鼠血清MDA明显升高(P<0.05),SOD、GSH明显降低(P<0.05),骨髓、小肠病理损伤加重。与辐射组比较,BBR组大鼠MDA血清水平明显下降(P<0.05),SOD、GSH明显升高(P<0.05),骨髓、小肠病理损伤减轻,高剂量组更为明显。结论小檗碱对辐射损伤大鼠具有一定的保护作用,机制可能是提高抗氧化物质的活性,增强对自由基的清除能力,从而减轻自由基造成的氧化损伤。Objective To investigate the protective effect of berberine(BBR)against ionizing radiation injury in rats and its mechanism of action.Methods Sprague-Dawley rats were divided into seven groups:normal control group,1-Gy radiation group,1-Gy radiation plus low-dose BBR(50 mg/kg)group,1-Gy radiation plus high-dose BBR(150 mg/kg)group,3-Gy radiation group,3-Gy radiation plus low-dose BBR(50 mg/kg)group,and 3-Gy radiation plus high-dose BBR(150 mg/kg)group.All the groups except the normal control group were exposed to external irradiation with a medical electron linear accelerator,followed by BBR administration by gavage for consecutive ten days.The serum levels of superoxide dismutase(SOD),reduced glutathione(GSH),and malondialdehyde(MDA)were measured by using the micromethod.The pathological changes of the bone marrow and small intestine were observed with HE staining.Results Compared with the normal control group,the radiation groups showed significantly increased MDA levels(P<0.05),significantly decreased SOD and GSH levels(P<0.05),and more severe pathological damage of the bone marrow and small intestine.Compared with the radiation groups,the BBR groups showed significantly decreased MDA levels(P<0.05),significantly increased SOD and GSH levels(P<0.05),and reduced pathological damage to the bone marrow and small intestine,which were more marked in the high-dose BBR group.Conclusion BBR has a certain protective effect against radiation injury in rats,which may be through increasing the activity of antioxidant substances,enhancing free radical clearance,and thereby alleviating free radicals-caused oxidative damage.

关 键 词：小檗碱 辐射损伤 SD大鼠 自由基 

分 类 号：X591[环境科学与工程—环境工程]