榆苋方保留灌肠对大肠湿热型溃疡性结肠炎大鼠PXR/NF-κB信号通路及炎症因子的调控作用研究  

Study on the regulatory effect of Yuxian Formula on retention enema on PXR/NF-κB signaling pathway and inflammatory factors in rats with ulcerative colitis of damp-heat type in large intestine

作　　者：杨振斌[1] 王庆娜 杨超[1] 邱伟[1] 陈莉丽[1] 夏文娟[1] 刘乐[1] 金超 YANG Zhen-bin;WANG Qing-na;YANG Chao;QIU Wei;CHEN Li-li;XIA Wen-juan;LIU Le;JIN Chao(Spleen and Stomach Diseases Department,Yangzhou Hospital Affiliated to Nanjing University of Traditional Chinese Medicine,Yangzhou 225009,China)

机构地区：[1]南京中医药大学附属扬州医院脾胃病科,扬州225009

出　　处：《北京中医药》2023年第8期847-851,共5页Beijing Journal of Traditional Chinese Medicine

基　　金：扬州市重点研发项目(社会发展)(YZ2020126);扬州市方晓华名中医工作室项目(2020扬州市人才办)。

摘　　要：目的观察榆苋方保留灌肠对大肠湿热型溃疡性结肠炎(ulcerative colitis,UC)大鼠结肠组织孕烷X受体(PXR)、核因子κB p65(NF-κBp65)蛋白表达以及白细胞介素-10(IL-10)、肿瘤坏死因子-α(TNF-α)及转化生长因子-β(TGF-β)水平的影响。方法将53只大鼠随机分为空白对照组、模型组、阳性对照组及榆苋方低剂量、中剂量、高剂量组,空白对照组8只,其余各组均9只。除空白对照组外,其余各组建立UC模型。造模成功后开始给药,阳性对照组给予0.02 g/mL柳氮磺胺吡啶(SASP)3 mL灌肠,榆苋方低、中、高剂量组分别给予0.09、0.18、0.36 g/mL榆苋方水煎剂3 mL灌肠,空白对照组、模型组给予等体积生理盐水灌肠。1次/d,连续给药14 d。实验结束后,收集结肠组织,对比各组组织学损伤评分、结肠黏膜损伤评分,Western blotting法检测结肠组织中PXR蛋白、NF-κB p65蛋白表达,ELISA法检测结肠组织IL-10、TNF-α、TGF-β水平。结果空白对照组结肠黏膜正常;模型组结肠黏膜糜烂严重,有大片状表浅溃疡;各治疗组与模型组相比结肠溃疡数量减少、面积缩小,黏膜损伤减轻。与空白对照组比较,模型组结肠黏膜损伤评分、组织学损伤评分、NF-κB p65蛋白表达、TNF-α、TGF-β水平高,结肠组织PXR蛋白表达、IL-10水平低(P<0.01);与模型组比较,各治疗组以上评分、NF-κB p65蛋白表达、TNF-α、TGF-β、TNF-α、TGF-β水平均低,PXR蛋白表达、IL-10水平高(P<0.05,P<0.01);榆苋方高剂量组以上评分、NF-κB p65蛋白表达均低于榆苋方低、中剂量组,PXR蛋白表达、IL-10水平高于榆苋方低、中剂量组(P<0.05,P<0.01)。榆苋方高剂量组以上指标与阳性对照组比较差异无统计学意义(P>0.05)。结论榆苋方保留灌肠可修复大肠湿热型UC大鼠肠黏膜损伤,并对PXR/NF-κB信号通路及炎症因子有调控作用。Objective To observe the expression of pregnane X receptor(PXR),nuclear factorκB p65(NF-κBp65),interleukin-10(IL-10),tumor necrosis factor-α(TNF-α)and transforming growth factor-β(TGF-β)in colon tissue of rats with ulcerative colitis(UC)of large intestine damp-heat type caused by retention enema with Yuxian Formula.Methods 53 rats were randomly divided into 6 groups,that is blank control group,model group,positive control group,low dose treatment group,medium dose treatment groupand high dose treatment group with,8 in blank control groupand 9 in other groups.Except the blank control group,the other groups were established ulcerative colitis models.After successful modeling,the drug was given.The positive control group was given 0.02 g/mL sulfasalazine(SASP)3 mL enema,and the low,medium and high dosage groups were given 0.09 g/mL,0.18 g/mL and 0.36 g/mL YuAmaran Decoction 3 mL enema respectively,while the blank control group and model group were given the same volume of normal saline enema,once a day for 14 days.After the experiment finished,the colon tissue was collected,the histological injury score and colonic mucosal injury score of each group were compared.The expressions of PXR protein and NF-κB p65 protein in colon tissue were detected by Western blotting,and the levels of IL-10,TNF-αand TGF-βin colon tissue were detected by ELISA.Results The colon mucosa in blank control group was normal.In the model group,the colonic mucosa was seriously eroded and there were large superficial ulcers.Compared with the model group,the number and area of colonic ulcers in each treatment group were reduced,and the mucosal injury was alleviated.Compared with the blank control group,the scores of colonic mucosal injury,histological injury,NF-κB p65 protein expression,TNF-αand TGF-βlevels in the model group were higher,while the expressions of PXR protein and IL-10 in the colon tissue were lower(P<0.01).Compared with the model group,the scores above,the expression of NF-κB p65 protein,the levels of TNF-α,TGF-β,TN

关 键 词：榆苋方 大肠湿热型 溃疡性结肠炎 炎症 孕烷X受体 核因子κB p65 大鼠 

分 类 号：R285.5[医药卫生—中药学]