病毒REV与ALV-J经鸡胚垂直传播共感染对雏鸡的协同致病性  

Synergistic Pathogenicity of REV and ALV-J in Chicks by Vertical Transmission of Chicken Embryos

作　　者：马凌遥 王一新[1] 赵鹏[1] 常爽[1] 蔡曼珊 MA Lingyao;WANG Yixin;ZHAO Peng;CHANG Shuang;CAI Manshan(College of Animal Science and Technology,Shandong Agricultural University,Tai’an 271018,China;Institute of Animal Science,Guangdong Academy of Agricultural Sciences/State Key Laboratory of Livestock and Poultry Breeding,Guangzhou 510640,China)

机构地区：[1]山东农业大学动物科技学院,山东泰安271018 [2]广东省农业科学院动物科学研究所/猪禽种业全国重点实验室,广东广州510640

出　　处：《广东农业科学》2023年第10期141-148,共8页Guangdong Agricultural Sciences

基　　金：广东省重点领域研发计划(2020B020222001)。

摘　　要：【目的】J亚群禽白血病病毒(Avian leukosis virus subgroup J,ALV-J)和禽网状内皮组织增殖病毒(Reticuloendotheliosis virus,REV)均可通过鸡胚垂直传播,且两者在鸡胚中的自然共感染已有报道,但两者经鸡胚共感染的致病性未有研究。建立ALV-J与REV垂直传播共感染的实验动物模型,研究ALV-J与REV垂直传播共感染的致病作用及其对禽白血病净化检测的影响,以期为推动多病原协同净化提供参考。【方法】通过鸡胚接种方式构建分别携带ALV-J、REV及两者共感染的雏鸡感染模型,通过孵化率、死亡率、体重、胎粪ALV-p27抗原检出率、病毒血症阳性率等指标分析REV与ALV-J在垂直传播中共感染的致病性。【结果】ALV-J+REV共感染组鸡胚孵化率为65.71%,低于单感染组和空白组(CK);共感染组死亡率高于单感染组和CK。7日龄时CK、ALV-J组、REV组、ALV-J+REV组雏鸡平均体重分别为59.25、50.83、49.67、43.78 g,共感染加重了对雏鸡生长的抑制。ALV血浆病毒分离结果显示,ALV-J组、ALV-J+REV组阳性率在各日龄均为100%,CK均为0%。采集肛拭子检测ALV-p27抗原结果显示,ALV-J+REV组雏鸡阳性率在1、3、7、14日龄分别为91.30%、94.12%、90.90%和100%,肛拭子的ALV-J p27阳性检出率低于血浆病毒分离。比较免疫器官发育指数结果显示,ALV-J和REV单感染均导致雏鸡免疫器官萎缩,共感染加重免疫器官萎缩程度。免疫器官病毒载量显示,REV与ALV-J共感染促进了ALV-J在免疫器官中的增殖,同时ALV-J也促进了REV在免疫器官中的增殖。【结论】相对于ALV-J和REV单感染,两者共感染降低了鸡胚孵化率、增加了雏鸡死亡率、增强了对雏鸡生长的抑制作用,对诱导胸腺、脾脏和法氏囊等免疫器官萎缩的影响更加显著,且两者在共感染过程中对彼此复制均有促进作用。【Objective】Avian leukosis virus subgroup J(ALV-J)and reticuloendotheliosis virus(REV)can be transmitted vertically through chicken embryos,and there have been reports of chicken embryos that are co-infected with the two viruses,however,the pathogenicity of co-infection between the two through chicken embryos has not been studied.An experimental animal model of vertical transmission of ALV-J and REV was established to study the pathogenicity of vertical transmission of ALV-J and REV and its effect on the purification test of avian leukaemia,with a view to providing a reference for the promotion of the synergistic purification of multiple pathogens.【Method】An infection model for chicks carrying ALV-J,REV and co-infected with the two viruses was constructed respectively by means of chicken embryo inoculation,and the pathogenicity of co-infection of REV and ALV-J in vertical transmission was analyzed by the indexes of hatching rate,mortality rate,body weight,fetal-feces ALV-p27 antigen detection rate,and viraemia positive rate.【Result】The hatching rate of the chicken embryos in the co-infection group of ALV-J+REV was 65.71%,which was lower than that in the mono-infection group and CK;the mortality rate in the co-infection group was higher than that in the mono-infection group and CK.The average weights of chicken in the CK,ALV-J group,REV group and ALV-J+REV group were 59.25,50.83,49.67,43.78 g,indicating that co-infection increased the inhibition of growth.The results of ALV plasma virus isolation showed that the positive rates of ALV-J group and ALV-J+REV group were 100%at each day age,while the rate of CK was 0%.The results of anal swabs collected for the detection of ALV-27 antigen showed that the positive rates of ALV-J+REV group were 91.30%,93.75%,90.90%and 100%at day age(s)of 1,3,7 and 14,respectively,and the positive detection rate of ALV-J p27 in anal swabs was lower than that of plasma virus isolation.Comparison of the results of the immune organ development showed that,both ALV-J mono-infection

关 键 词：禽白血病病毒(ALV) 禽网状内皮组织增殖病毒(REV) 垂直传播 共感染 致病性 净化 

分 类 号：S858.31[农业科学—临床兽医学]