西洋参改善睡眠有效成分的研究  

Study on the Effective Components of Panax quinquefolium for Improving Sleep

作　　者：闫晓军 李阳 YAN XiaoJun;LI Yang(School of Chemical Engineering,Northwest University,Xi′an 710069,China;Biotech.&Biomed.Research Institute,Northwest University,Xi′an 710069,China)

机构地区：[1]西北大学化工学院,西安710069 [2]西北大学生物医药研究院,西安710069

出　　处：《世界睡眠医学杂志》2023年第7期1673-1676,共4页World Journal of Sleep Medicine

摘　　要：目的:探究西洋参改善睡眠的有效成分。方法:通过直接睡眠试验、延长戊巴比妥钠睡眠时间试验、戊巴比妥钠阈下剂量催眠试验、巴比妥钠睡眠潜伏期试验,评价西洋参水提物、西洋参皂苷、西洋参多糖对小鼠睡眠作用的影响。结果:西洋参水提物、西洋参皂苷、西洋参多糖各剂量组对正常小鼠均无直接睡眠作用。西洋参皂苷各剂量组均可以延长戊巴比妥钠诱导的小鼠睡眠时间,差异有统计学意义(P<0.05或P<0.01),增加戊巴比妥钠阈下催眠剂量入睡小鼠只数,差异有统计学意义(P<0.05或P<0.01),缩短小鼠的睡眠潜伏期,差异有统计学意义(P<0.05或P<0.001)。西洋参皂苷、西洋参水提物高剂量组在延长戊巴比妥钠睡眠时间、缩短巴比妥钠睡眠潜伏期方面效果优于西洋参多糖组,差异有统计学意义(P<0.05)。此外,西洋参皂苷高剂量组小鼠下丘脑及血清中的5-羟色胺(5-HT)及γ-氨基丁酸(GABA)含量均显著高于对照组,差异有统计学意义(P<0.01或P<0.001),多巴胺(DA)含量均显著低于对照组,差异有统计学意义(P<0.01)。结论:西洋参水提物、西洋参皂苷、西洋参多糖均可在一定程度上改善小鼠睡眠,西洋参皂苷是改善睡眠的主要功效成分。Objective:To explore the effective components of Panax quinquefolium in improving sleep.Methods:Through direct sleep test,extended pentobarbital sodium sleep time test,pentobarbital sodium subthreshold dose hypnosis test,and barbital sodium sleep latency test,evaluate the effects of Panax quinquefolium water extract,Panax quinquefolium saponin,Panax quinquefolium polysaccharide on sleep in mice.Results:Panax quinquefolium water extract,Panax quinquefolium saponin and Panax quinquefolium polysaccharide in each dose group had no direct sleep effect on normal mice.Panax quinquefolium saponin in each dose group could prolong the sleep time of mice induced by pentobarbital sodium(P<0.05 or P<0.01),increase the number of mice falling asleep at the subthreshold hypnotic dose of pentobarbital sodium(P<0.05 or P<0.01),and significantly shorten the sleep latency of mice(P<0.05 or P<0.01).The effect of Panax quinquefolium saponin and Panax quinquefolium water extract high-dose groups was better than that of Panax quinquefolium polysaccharide group in prolonging sleep time of pentobarbital sodium,and shortening sleep latency of sodium barbital(P<0.05).In addition,5-hydroxytryptamine(5-HT)andγ-the content of aminobutyric acid(GABA)of Panax quinquefolium saponin group was significantly higher than that of the control group(P<0.01 or P<0.001),the content of dopamine(DA)was significantly lower than that in the control group(P<0.01).Conclusion:Panax quinquefolium water extract,Panax quinquefolium saponin and Panax quinquefolium polysaccharide can improve the sleep of mice to a certain extent,and Panax quinquefolium saponin is the main effective ingredient to improve sleep.

关 键 词：西洋参水提物 西洋参皂苷 西洋参多糖 睡眠 戊巴比妥钠 巴比妥钠 

分 类 号：R338.63[医药卫生—人体生理学]