柳树离体再生及遗传转化体系研究进展  

In Vitro Regeneration and Genetic Transformation System of Willow:Research Progress

作　　者：刘源 李际红 秦光华[2] 刘翠双 孙茂桐 牛牧歌 王锦楠 LIU Yuan;LI Jihong;QIN Guanghua;LIU Cuishuang;SUN Maotong;NIU Muge;WANG Jinnan(Key Laboratory of Forest Cultivation of the Lower Reaches of the Yellow River,National Forestry and Grassland Administration/National Positioning Observation and Research Station of Taishan Forest Ecosystem/College of Forestry,Shandong Agricultural University,Tai'an,Shandong 271018;Shandong Academy of Forestry Sciences,Jinan 250014)

机构地区：[1]山东农业大学林学院/山东泰山森林生态系统国家定位观测研究站/黄河下游森林培育国家林业和草原局重点实验室,山东泰安271018 [2]山东省林业科学研究院,济南250014

出　　处：《中国农学通报》2023年第29期32-38,共7页Chinese Agricultural Science Bulletin

基　　金：山东省农业良种工程项目(山东省科技厅)“功能性柳树新品种选育及应用”(2021LZGC005-02)。

摘　　要：柳树因其药用价值、生态修复能力及作为生物能源的潜力而受到关注。近些年已培育出许多优良品种,并在柳属植物基因组中定位到与经济性状及抗逆性相关的候选基因,建立离体再生及遗传转化体系可以获得大量优质柳树苗,并对候选基因进行功能研究。为了更清晰地认识影响柳树再生及遗传转化的不同因素,本文对柳树离体再生和遗传转化的研究进展进行了总结,主要包括柳属不同种初代培养、继代增殖培养、生根培养的最佳培养基的筛选,诱导愈伤组织最佳的培养基、激素、外植体类型的筛选,遗传转化时最佳的农杆菌的类型、浓度、侵染时间、预培养和共培养时间及选择压等因素的筛选。目前柳树的愈伤组织再生和遗传转化仍存在难点,本研究对其未来进一步深入研究方向进行了展望,以期对柳树的离体再生及遗传转化体系建立提供参考,为后续柳树基因工程研究及柳树的遗传改良奠定基础。Willow has attracted attention because of its medicinal value,ecological restoration capacity and potential as a bioenergy source.In recent years,many excellent willow varieties have been cultivated,and candidate genes related to economic traits and stress resistance have been identified in the genome of Salix.A large number of high-quality willow saplings can be obtained by establishing in vitro regeneration and genetic transformation system,and the function of candidate genes can be studied.In order to have a clear understanding of different factors affecting willow regeneration and genetic transformation,this paper reviewed the research progress of willow in vitro regeneration and genetic transformation,including the selection of optimal medium for primary culture,subculture and rooting culture of different Salix species,the selection of optimal medium,hormone and explant type for callus induction,the effects of Agrobacterium type,concentration,infection time,preculture and co-culture time on genetic transformation,and the optimal concentration of kanamycin for selecting efficiency of genetic transformation.At present,there are still some difficulties in the regeneration and genetic transformation of willow callus.In this study,further research directions of willow callus regeneration and genetic transformation were discussed to provide reference for establishing the in vitro regeneration and genetic transformation system of willow,and forming a foundation for the subsequent genetic engineering research and genetic improvement of willow.

关 键 词：柳树 离体再生 遗传转化 

分 类 号：S722.37[农业科学—林木遗传育种]