基于整合药理学平台的当归补血汤治疗贫血分子作用机制研究  

Study on the Molecular Mechanism of Danggui Buxue Tang in Treating Anemia Based on TCMIP

作　　者：侯敏娜[1] 侯少平[1] 逯莉[1] 王飞娟[1] 许海燕[1] 刘艳红[1] HOU Minna;HOU Shaoping;LU Li;WANG Feijuan;XU Haiyan;LIU Yanhong(Shaanxi Institute of International Trade&Commerce,Xianyang 712046,China)

机构地区：[1]陕西国际商贸学院,陕西咸阳712046

出　　处：《西部中医药》2023年第11期8-13,共6页Western Journal of Traditional Chinese Medicine

基　　金：国家科技部重大新药创制科技重大专项(2018ZX09721005-009-013);陕西省重点研发计划(2020SF-323);陕西国际商贸学院中药质量标志物创新团队(SSY18TD02);陕西省大学生创新创业训练计划项目(S202013123043,S202013123037,S202013123045,S202013123023);陕西省教育厅2021年度青年创新团队科研计划项目(21JP012)。

摘　　要：目的:借助整合药理学平台研究当归补血汤治疗贫血的分子作用机制。方法:依托中医药整合药理学平台(integrative pharmacology-based research platform of traditional Chinese medicine,TCMIP)V2.0,构建当归补血汤治疗贫血的“方剂-中药材-成分-疾病-靶标-通路”多维关系网络图,预测其治疗贫血疾病的关键靶标和作用通路,揭示其分子作用机制。结果:当归补血汤中与贫血疾病相关的化学成分包括谷甾醇、叶酸、华良姜素、豆甾醇、琥珀酸、叶酸、癸二酸、壬二酸8种,核心靶标176个,共有靶标9个;前20位核心靶标和通路中多数靶标与癌性贫血发生有关,如Akt1、CCND1、ABL1、EPOR、GATA1、SMAD4等可能通过细胞增殖正调节、细胞增长负调节、蛋白激酶b信号蛋白正调节、细胞分化等通路起抑制癌细胞增殖、增长、分化及凋亡等作用;而NFKB1、HSP90AA1、TFRC、TF、NDUFV1、NDUFS1等靶标可能通过细胞因子介导的信号通路、Nf-kappab转录因子正调节、线粒体呼吸链组装、红细胞分化、促红细胞生成素介导信号通路、肽丝氨酸磷酸化正调节等途径,以提高免疫力、补充营养、消除炎症等方式治疗缺铁性贫血、炎性贫血、心脑缺血、糖尿病并发症及改善营养不良性等的贫血。结论:当归补血汤可通过多靶点、多通路治疗缺铁性贫血、心脑缺血、癌性贫血、炎性贫血、营养不良性贫血及糖尿病、高脂血症等引起的贫血。Objective:To study the molecular mechanism of Danggui Buxue Tang in the treatment of anemia based on TCMIP.Methods:Based on TCMIP V2.0,the multi-dimensional relationship network diagram of"prescription-Chinese medicine-composition-disease-target-pathway"was constructed to predict the key targets and action pathways of Danggui Buxue Tang in treating anemia,and reveal its molecular mechanism of action.Results:Eight kinds of anemia-related chemical components in Danggui Buxue Tang were sitosterol,3-hydroxycoumarin folic acid,kumatakenin,stigmasterol,succinic acid,folic acid,sebacic acid and azelaic acid,there were 176 core targets and nine common targets;the top 20 core targets and most of the targets in the pathways were related to cancer-related anemia,for instance,the targets of Akt1,CCND1,ABL1,EPOR,GATA1,SMAD4 and others could inhibit the proliferation,growth,differentiation and apoptosis of cancer cells possibly through positive regulation of cell proliferation,negative regulation of cell growth,positive regulation of protein kinase B signal protein and cellular differentiation;while targets including NFKB1,HSP90AA1,TFRC,TF,NDUFV1,NDUFS1,etc,could treat iron deficiency anaemia,inflammatory anaemia,cardiac and cerebral ischaemia,diabetic complications and malnutritional anaemia by boosting immunity,supplementation and eliminating inflammation,possibly through cytokine-mediated signaling pathway,positive regulation of Nf-kappab transcription factor,mitochondrial chain assembly,red blood cell differentiation,hemopoietin-mediated signaling pathway,positive regulation of peptide serine phosphorylation,etc.Conclusion:Danggui Buxue Tang could be used to treat iron deficiency anaemia,cardiac and cerebral ischaemia,cancerous anemia,inflammatory anaemia,malnutritional anaemia,and anaemia induced by diabetes and hyperlipemia.

关 键 词：贫血 当归补血汤 分子作用机制 整合药理学 

分 类 号：R259[医药卫生—中西医结合]