益气养阴方联合电针对产后压力性尿失禁大鼠CXCR4/COX-2信号通路的影响  

The Effects of Benefiting-Qi Nourishing-Yin Prescription Combined with Electroacupuncture on CXCR4/COX-2 Signal Pathway in Rats with Postpartum Urinary Incontinence

作　　者：徐静 王旭[1] 房桂英[1] 黄丽霞[1] 魏旭静 XU Jing;WANG Xu;FANG Guiying;HUANG Lixia;WEI Xujing(Department of Obstetrics and Gynecology,the First Hospital of Hebei Medical University,Shijiazhuang 050051,China)

机构地区：[1]河北医科大学第一医院妇产科,河北石家庄050051

出　　处：《西部中医药》2023年第11期33-37,共5页Western Journal of Traditional Chinese Medicine

基　　金：河北省医学科学研究课题计划项目(20200123)。

摘　　要：目的:探究益气养阴方联合电针对产后压力性尿失禁(postpartum urinary incontinence,PPUI)大鼠趋化因子受体4(chemokine receptor 4,CXCR4)/环氧合酶2(cyclooxygenase,COX-2)信号通路的作用机制。方法:将大鼠随机分为空白组、PPUI组、益气养阴组和益气养阴+电针组,制备PPUI大鼠模型;检测4组大鼠收缩力/肌重比值、血清乳酸脱氢酶(lactate dehydrogenase,LDH)和心肌酶肌酸激酶(creatine kinase,CK)含量;HE染色检测4组大鼠肛提肌组织病理学变化;蛋白质印迹法检测4组大鼠尿道组织CXCR4和COX-2蛋白表达。结果:与空白组相比,大鼠最大膀胱容量和漏尿压力点PPUI组降低(P<0.05),益气养阴组和益气养阴+电针组升高,益气养阴+电针组升高明显(P<0.05);与空白组相比,收缩力/肌重比值PPUI组降低(P<0.05),益气养阴组和益气养阴+电针组较PPUI组增加,且益气养阴+电针组增加更明显(P<0.05);与空白组相比,LDH和CK含量PPUI组升高(P<0.05),益气养阴组和益气养阴+电针组低于PPUI组(P<0.05)。与空白组相比,PPUI组CXCR4蛋白减少,COX-2蛋白增多(P<0.05),益气养阴组和益气养阴+电针组CXCR4蛋白较PPUI组增多,COX-2蛋白较PPUI组减少(P<0.05),益气养阴+电针组CXCR4蛋白较益气养阴组增加,COX-2蛋白较益气养阴组减少(P<0.05)。结论:益气养阴方联合电针可能通过抑制PPUI大鼠血清LDH和CK表达,增加PPUI大鼠CXCR4表达,抑制COX-2表达,增加盆底肌肌肉收缩功能,治疗PPUI。Objective:To survey the mechanism of benefiting-Qi nourishing-Yin prescription in combination with electroacupuncture on CXCR4/COX-2 signal pathway in rats suffering postpartum urinary incontinence(PPUI).Methods:All the rats were randomized into the blank group,PPUI group,benefiting-Qi nourishing-Yin group,benefiting-Qi nourishing-Yin+electroacupuncture group,to establish PPUI rat models;to detect contraction force/muscle weight ratio of the rats in four groups.The contents of LDH and CK;HE staining is applied to detect histopathological changes of musculi levator ani of the rats in four groups;Western blotting method to measure the expressions of CXCR4 and COX-2 protein in urethral tissue of the rats in four groups.Results:Compared with the blank group,maximum bladder capacity and leakage pressure point of rats in PPUI group were decreased(P<0.05),after intervention,maximum bladder capacity and leakage pressure point of benefiting-Qi nourishing-Yin group and benefiting-Qi-Yin+electroacupuncture group were significantly restored,and the recovery in benefiting-Qi nourishing-Yin+electroacupuncture group was the best(P<0.05);compared with the blank group,the ratio of contractile force to muscle weight in the PPUI group was lowered(P<0.05),the ratio in benefiting-Qi nourishing-Yin group and benefiting-Qi nourishing-Yin+electroacupuncture group was significantly higher than that in the PPUI group,and the increase in benefiting-Qi nourishing-Yin+electroacupuncture group was more noticeable(P<0.05);compared with the blank group,the contents of LDH and CK increased in the PPUI group(P<0.05),benefiting-Qi nourishing-Yin group and benefiting-Qi nourishing-Yin+electroacupuncture group were lower than the PPUI group(P<0.05).Compared with the blank group,CXCR4 protein reduced while COX-2 protein increased in the PPUI group(P<0.05),the increase of CXCR4 protein in benefiting-Qi nourishing-Yin group and benefiting-Qi nourishing-Yin+electroacupuncture group were more than that in the PPUI group,COX-2 protein reduction was more o

关 键 词：产后压力性尿失禁 CXCR4/COX-2信号通路 益气养阴方 电针 大鼠 

分 类 号：R246.3[医药卫生—针灸推拿学]