正交-满意度函数优化秦艽黄酮和环烯醚萜苷超声共提取条件  

Optimization of Co-extraction Technology of Flavonoids and Iridoid Glycosides from Qinjiao by Orthogonal Design-Desirability

作　　者：刘艳红[1] 侯敏娜[1] 彭修娟[1] 王珊[1] 王青[1] 许海燕[1] LIU Yanhong;HOU Minna;PENG Xiujuan;WANG Shan;WANG Qing;XU Haiyan(Department of medicine,Shaanxi Institute of International Trade&Commerce,Xi'an 712046,China)

机构地区：[1]陕西国际商贸学院医药学院,陕西西安712046

出　　处：《西部中医药》2023年第11期52-56,共5页Western Journal of Traditional Chinese Medicine

基　　金：陕西省教育厅专项科研计划项目(17JK0948);陕西省重点研发计划(2017SF-369)。

摘　　要：目的:优化秦艽黄酮和环烯醚萜苷超声提取工艺条件。方法:以秦艽黄酮和环烯醚萜苷提取率为考察指标,以乙醇体积分数、料液比、提取温度及提取时间为影响因素,进行单因素试验;采用正交-满意度优化秦艽黄酮和环烯醚萜苷提取工艺。结果:优选出秦艽中黄酮及环烯醚萜苷提取的最佳工艺条件为乙醇体积分数60%,料液比1∶30 g/mL,提取温度60℃,提取时间40 min,在此条件下的提取率黄酮为2.20%,环烯醚萜苷为9.32%,平均满意度为0.997,RSD=0.28%。结论:优选的条件方法简便、高效、易于实现,适合秦艽中黄酮与环烯醚萜苷的提取。Objective:To optimize the ultrasound extraction technology of flavonoids and iridoid glycosides from Qinjiao(Gentianae macrophyllae radix).Methods:Single factor test was performed by taking the extraction percentage of flavonoids and iridoid glycosides as the investigation indexes,ethanol volume fraction,liquid-solid ratio,extraction temperature and extraction time as the influencing factors;and the extraction technology of flavonoids and iridoid glycosides from Qinjiao was optimized by orthogonal design-desirability.Results:The best extraction conditions for flavonoids and iridoid glycosides from Qinjiao were as follows:ethanol volume fraction of 60%,liquid-solid ratio of 1:30 g/mL with extraction temperature 60℃and extraction time 40 min.Under these conditions,the extraction percentages of flavonoids and iridoid glycosides were 2.20%and 9.32%,average desirability of the confirmatory test was 0.997,RSD=0.28%.Conclusion:The extraction conditions,convenient,efficient and realizable,are suitable for the extraction of flavonoids and iridoid glycosides from Qinjiao.

关 键 词：秦艽 黄酮 环烯醚萜苷 提取工艺 正交-满意度 

分 类 号：R284.2[医药卫生—中药学]