活体荧光成像示踪移植干细胞的研究进展  

Advances in Tracking Transplanted Stem Cells by Intravital Fluorescence Imaging

作　　者：吕尚瑞 周逸男 李琼 肖大江[1] LYU Shangrui;ZHOU Yinan;LI Qiong;XIAO Dajiang(Department of Otorhinolaryngology Head and Neck Surgery,Affiliated Wuxi No.2 People′s Hospital of Nanjing Medical University,Wuxi 214000,China;Nanjing Medical University,Nanjing 210000,China;Department of Neurosurgery,Affiliated Wuxi Clinical College of Nantong University,Wuxi 214000,China)

机构地区：[1]南京医科大学附属无锡第二医院耳鼻咽喉头颈外科,江苏无锡214000 [2]南京医科大学,南京210000 [3]南通大学无锡临床学院神经外科,江苏无锡214000

出　　处：《医学综述》2023年第11期2234-2239,共6页Medical Recapitulate

基　　金：无锡市卫生计生委科研项目(Z201802)。

摘　　要：荧光成像是一项以激发荧光探针为手段的生物成像技术,它可以在活生物体内的细胞水平上对生物过程进行可视化和量化。在以干细胞为基础的再生医学领域,利用荧光团标记干细胞,可以实时、无创监测移植干细胞在动物体内的生物学活动,这对明确干细胞再生过程中的机制以获得更好的治疗效果至关重要。尤其是近年对近红外光谱区域的探索和新型荧光探针的开发利用,更是给荧光成像领域带来了革命性的变化。开发高灵敏度和多功能的荧光探针将是未来的研究方向,以更好地示踪移植干细胞在体内的活动。Fluorescence imaging is a bioimaging technology that uses activated fluorescent probes to visualize and quantify biological processes at the cellular level in living organisms.In the field of stem cell-based regenerative medicine,the use of fluorophores to label stem cells can real-time and non-invasively monitor the biological activities of transplanted stem cells in animals,which is crucial for clarifying the mechanism of stem cell regeneration for better therapeutic effects.Especially in recent years,the exploration of the near-infrared spectral region and the development and utilization of new fluorescent probes have brought revolutionary changes in the field of fluorescence imaging.The future research direction will be to develop highly sensitive and multifunctional fluorescent probes to better track the activities of transplanted stem cells in vivo.

关 键 词：荧光成像 干细胞 荧光团 近红外光谱 

分 类 号：R318.51[医药卫生—生物医学工程]