Pi9基因标记辅助选择改良水稻不育系金23A稻瘟病抗性  

Improving blast resistance of rice CMS line Jin23A using marker-assisted selection of the Pi9 gene

作　　者：车凡昊 黄俊[1] 邹玉莹 邓吉奇 周庚 姚威 黄浩 黄希来 易晓璇 谌强 李家鑫 刘金灵[1,2,3] 刘雄伦 CHE Fanhao;HUANG Jun;ZOU Yuying;DENG Jiqi;ZHOU Geng;YAO Wei;HUANG Hao;HUANG Xilai;YI Xiaoxuan;CHEN Qiang;LI Jiaxin;LIU Jinling;LIU Xionglun(College of Agronomy,Hunan Agricultural University,Changsha,Hunan 410128,China;Crop Gene Engineering Key Laboratory of Hunan Province,Changsha,Hunan 410128,China;Rice and Rapeseed Disease Resistance Breeding Key Laboratory of Hunan Province,Changsha,Hunan 410128,China)

机构地区：[1]湖南农业大学农学院,湖南长沙410128 [2]作物基因工程湖南省重点实验室,湖南长沙410128 [3]水稻油菜抗病育种湖南省重点实验室,湖南长沙410128

出　　处：《湖南农业大学学报（自然科学版）》2023年第5期503-508,515,共7页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：湖南省科技重大专项(2021NK1001);国家自然科学基金项目(31972256)。

摘　　要：以携带广谱持久的抗稻瘟病基因Pi9的籼稻品系75–1–127为供体亲本,以保持系金23B及其不育系金23A为受体亲本,采用Pi9基因共显性标记CoInDF1R2进行MAS回交育种,以改良三系不育系金23A及其保持系金23B的稻瘟病抗性;用25份稻瘟菌代表性菌株进行室内苗瘟接种,观察苗瘟抗性表现。结果显示:金23A和金23B的抗性频率仅为28%,而75–1–127的抗性频率为92%;田间病圃抗性鉴定结果显示,75–1–127高抗苗瘟,而金23A和金23B受体亲本均高感苗瘟;共显性标记CoInDF1R2在75–1–127基因组上扩增出大小为354 bp的PCR产物,对金23A或金23B基因组的扩增产物大小约470 bp,说明该标记在2个亲本间的多态性明显且稳定;利用CoInDF1R2开展连续多年MAS回交育种实践,培育出了农艺性状优良、丰产性较好的抗病不育系金23A–Pi9–1及抗病保持系金23B–Pi9–1,为三系法杂种优势利用提供了新的抗稻瘟病亲本材料。To improve blast resistance of the rice cytoplasmic male sterile(CMS)line Jin23A and its maintainer Jin23B,the indica rice line 75-1-127 carrying a broad-spectrum and durable blast resistance gene Pi9 was used as the donor parent,and Jin 23B and Jin 23A were used as the acceptor parents,a co-dominant marker,CoInDF1R2,was developed according to Pi9 sequence,then was employed in marker-assisted selection breeding practice.Greenhouse inoculation assay was performed using twenty-five Magnaporthe oryzae isolates collected from different rice areas.The results showed that the Pi9 gene donor rice line 75-1-127 showed much higher resistance frequency(92%)than those of two acceptor parents Jin23A and Jin23B(28%).Accordingly,75-1-127 showed high-level seedling blast resistance but highly susceptible for the receptors in natural nursery.CoInDF1R2 showed clear and stable co-dominant polymorphism between the donor and acceptor parents,amplifying a 354 bp DNA band from genome of 75-1-127,while the PCR product from either genome of Jin23A or Jin23B was about 470 bp.An improved CMS line Jin23A-Pi9 and the corresponding maintainer Jin23B-Pi9 were created through consecutive backcross breeding practice by marker-assisted selection of the CoInDF1R2 marker.Then,Jin23A-Pi9-1 and Jin23B-Pi9-1 were screened as the goal CMS line and maintainer.This study had set the stage for developing elite hybrid rice varieties with superior blast resistance using the three-line method of heterosis utilization.

关 键 词：水稻 稻瘟病 抗性改良 分子标记辅助选择 Pi9基因 

分 类 号：S511.034[农业科学—作物学]