辣椒MADS-box基因家族的鉴定及表达分析  

Identification and expression analysis of the MADS-box gene family in Capsicum annuum

作　　者：杨光彬 王瑾 陈恺琳 单庆云 崔苏菲 熊程 邹学校 刘峰[2,3,4,5] YANG Guangbin;WANG Jin;CHEN Kailin;SHAN Qingyun;CUI Sufei;XIONG Cheng;ZOU Xuexiao;LIU Feng(Longping Branch,Hunan University,Changsha,Hunan 410125,China;Laboratory of Lingnan Modern Agriculture,Guangzhou,Guangdong 510642,China;College of Horticulture,Hunan Agricultural University,Changsha,Hunan 410128,China;ERC for Germplasm Innovation and New Variety Breeding of Horticultural Crops,Changsha,Hunan 410128,China;Key Laboratory for Vegetable Biology of Hunan Province,Changsha,Hunan 410128,China;College of Horticulture,Nanjing Agricultural University,Nanjing,Jiangsu 210095,China)

机构地区：[1]湖南大学隆平分院,湖南长沙410125 [2]岭南现代农业实验室,广东广州510642 [3]湖南农业大学园艺学院,湖南长沙410128 [4]园艺作物种质创新与新品种选育教育部工程研究中心,湖南长沙410128 [5]蔬菜生物学湖南省重点实验室,湖南长沙410128 [6]南京农业大学园艺学院,江苏南京210095

出　　处：《湖南农业大学学报（自然科学版）》2023年第5期558-566,共9页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家自然科学基金项目(32002058、32130097);国家自然科学基金联合基金项目(U19A202);湖南省科技创新计划项目(2021JC0007)。

摘　　要：利用辣椒的全基因组数据鉴定到104个MADS–box基因,对它们的理化性质、染色体定位、系统进化关系、蛋白保守基序和组织表达水平进行分析。结果表明:辣椒MADS–box基因家族各成员在染色体上的分布不均,理化性质差异较大,104个家族成员编码的氨基酸长度为100~567 aa,蛋白相对分子质量为11203.9~63559.7,蛋白理论等电点(PI)为4.63~10.46,系统进化树分析结果表明,辣椒MADS–box基因家族可分为2大类,与拟南芥和番茄的进化关系类似;组织表达水平分析结果表明,CaMADSs主要在花、果实和种子中表达,在叶片中表达量相对较低,推测MADS–box基因可能参与调控果实的发育和成熟。In this study,104 MADS-box genes were identified from pepper by using the whole genome data of pepper,and their physical and chemical properties,chromosome location,phylogenetic relationship,protein conserved motifs and tissue expression levels were analyzed.The results showed that the members of MADS-box gene family in pepper were uneven distribution on chromosomes and their physical and chemical properties were quite different.The length of amino acids encoded by 104 family members was 100-567 aa,the relative molecular masses of proteins were 11203.9-63559.7,and the theoretical isoelectric points(PI)of proteins were 4.63-10.46.Phylogenetic analysis showed that the MADS-box gene family of pepper could be divided into two categories,which were similar to the evolutionary relationship between Arabidopsis thaliana and tomato.The analysis of tissue expression level showed that CaMADSs were mainly expressed in flowers,fruits and seeds,and was less expressed in leaves,speculating that MADS-box gene might be involved in regulating fruit development and ripening.

关 键 词：辣椒 MADS–box基因家族 基因表达 

分 类 号：S641.3[农业科学—蔬菜学] Q786[农业科学—园艺学]