鸡传染性喉气管炎病毒GZNY202112的分离鉴定及其TK、gD基因的遗传进化分析  

Isolation and identification of avian infectious laryngotracheitis virus GZNY202112 and genetic evolution analysis of TK and gD genes

作　　者：孙芸 温贵兰[1,2] 徐飞 李波[3] 杨婷 胡璇 SUN Yun;WEN Gui-lan;XU Fei;LI Bo;YANG Ting;HU Xuan(College of Animal Science,Guizhou University,Guiyang 550025,China;Guizhou Provincial Animal Biological Products Engineering Technology Research Center,Guiyang 550016,China;Guizhou Livestock and Poultry Genetic Resources Management Station,Guiyang 550001,China)

机构地区：[1]贵州大学动物科学学院,贵州贵阳550025 [2]贵州省动物生物制品工程技术研究中心,贵州贵阳550016 [3]贵州省畜禽遗传资源管理站,贵州贵阳550001

出　　处：《中国兽医科学》2023年第10期1270-1278,共9页Chinese Veterinary Science

基　　金：2021年度贵州省科技支撑计划项目;贵州生态养殖鸡五种常发疫病防控技术研究与示范项目;黔科合支撑([2021]一般164);贵州省级农业科技支撑计划项目(黔科合支撑[2019]2286号);2022年省级“金课”《兽医免疫学》线上线下混合一流课程项目;贵州大学2020年省级教学内容和课程体系改革项目(GZJG20200035)。

摘　　要：贵州省某养鸡场两栋鸡舍中的蛋鸡疑似发生了鸡传染性喉气管炎病毒感染,为确定该病病因,引物按已发表的TK基因引物序列进行合成,从发病鸡采集的病料中扩增出1259 bp大小的片段;将阳性病料组织处理后接种LMH细胞,盲传3代后发现LMH细胞出现CPE现象,收集其病毒液对其进行PCR鉴定、生长曲线的测定以及动物回归试验,确定分离到一株ILTV毒株,并将其命名为GZNY202112株。为了进一步了解其遗传特征,根据GenBank发表的鸡传染性喉气管炎病毒TK基因、gD基因设计了特异性引物,使用DNAstar软件绘制遗传进化树。TK以及gD遗传进化分析结果显示该毒株与澳大利亚原产疫苗株CSW-1、V1-99亲缘关系最近,GZNY202112株可能与ILT疫苗株有关。In order to determine the cause of infectious laryngotracheitis virus infection,the laying hens in two chicken houses of a chicken farm in Guizhou province were suspected to have been infected with infectious laryngotracheitis virus.Primers were synthesized according to the published primers sequence of TK gene and 1259 bp fragments were amplified from the infected chicken materials;LMH cells were inoculated with positive samples after tissue treatment,CPE phenomenon was found in LMH cells after 3 generations of blind transmission,the virus was collected and identified by PCR,growth curve determination,and animal regression experiments to determine an ILTV strain,which was named GZNY202112 strain.To learn more about its genetic characteristics,specific primers were designed according to TK gene and gD gene of infectious laryngotracheitis virus of chicken published by Gen Bank,and DNAstar software was used to draw the genetic evolution tree.Evolution analysis showed of TK and gD genetic showed that this strain was closely related to the Australian vaccine strains CSW-1 and V1-99,and GZNY202112 might be related to the ILT vaccine strain.

关 键 词：鸡传染性喉气管炎病毒 分离鉴定 遗传分析 

分 类 号：S852.659.1[农业科学—基础兽医学]