森林革蜱Septin-2的原核表达及其免疫分析  

Prokaryotic expression and immune analysis of Septin-2in Dermacentor silvarum(Acari:Ixodoidea)

作　　者：车丽妍 陈则宇 苟梦扬 王彦龙 昝晓庆 任巧云[1] 刘光远[1] 罗金[1] CHE Li-yan;CHEN Ze-yu;GOU Meng-yang;WANG Yan-long;ZAN Xiao-qing;REN Qiao-yun;LIU Guang-yuan;LUO Jin(State Key Laboratory for Animal Disease Control and Prevention/Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China;College of Animal Sciences,Tarim University/Key Laboratory of Tarim Animal Husbandry Sciences and Technology,Xinjiang Production and Construction Corps,Aral 843300,China;School of Agriculture,Ningxia University,Yinchuan 750021,China)

机构地区：[1]中国农业科学院兰州兽医研究所动物疫病防控全国重点实验室,甘肃兰州730046 [2]塔里木大学动物科学学院新疆生产建设兵团塔里木畜牧科技重点实验室,新疆阿拉尔843300 [3]宁夏大学农学院,宁夏银川750021

出　　处：《中国兽医科学》2023年第10期1292-1297,共6页Chinese Veterinary Science

基　　金：国家寄生虫资源中心项目(2019-194-30);中国农业科学院科技创新工程项目(ASTIP);国家肉牛牦牛产业技术体系项目(CARS-37);甘肃省基础研究创新群体项目(22JR5RA024)。

摘　　要：为了了解森林革蜱Septin-2基因的结构、表达特征及生物学功能,通过对Septin-2基因进行克隆、原核表达及生物信息学分析,利用RT-qPCR方法检测该基因在森林革蜱不同发育时期以及不同组织间的表达丰度。结果显示,本实验室培养的森林革蜱庆阳株Septin-2基因中最大编码区为1281 bp,可编码大小为426 aa的蛋白,主要功能区基因大小为868 bp,与森林革蜱陕西株(KAH7952853,XP_037569269)的同一性最高,高达100%;森林革蜱Septin-2在卵巢中的表达丰度最高,且蜱饱血后的表达量明显高于饥饿状态的蜱,Septin-2基因能够表达37 ku的重组蛋白,并能被家兔抗森林革蜱血清识别。上述研究表明,Septin-2基因在蜱饱血及卵巢的发育过程中均表达。In order to understand the structure,expression characteristics and biological function of Septin-2 gene in Dermacentor silvarum,the possible functions of Septin-2 was resolved by cloning,prokaryotic expression and bioinformatics analysis,and the RT-q PCR was used to detect the expression abundance of the Septin-2 in different developmental stages and different tissues of D.silvarum.The results showed that the maximum coding domain size of Septin-2 gene was 1281 bp,and encoding426 amino acids size protein,and the main functional region gene was 868 bp in size.The identity of the gene was 100%with that of shaanxi strain(KAH7952853,XP_037569269).The abundance of Septin-2 expression was highest in the ovary,and was significantly higher after engorgement than that in un-engorgement ticks.The recombinant Septin-2 protein was 37 ku in molecular weight,and can be identified with rabbit anti-D.silvarum serum.This study indicated that Septin-2 was expressed in ticks engorgement and ovarian development.

关 键 词：森林革蜱 Septin-2基因 生物信息学 

分 类 号：S852.746[农业科学—基础兽医学]