基于指纹图谱结合化学计量学方法评价不同产地野生与栽培赤芍质量  

Quality evaluation of wild and cultivated Paeoniae Radix Rubra from different origins based on fingerprint combined with stoichiometry methods

作　　者：于佳禾 金重先 刘金凤 魏梅 陈向东 石晋丽[1] YU Jiahe;JIN Zhongxian;LIU Jinfeng;WEI Mei;CHEN Xiangdong;SHI Jinli(School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China;Guangdong YiFang Pharmaceutical Co.Ltd.,Foshan 528000,Guangdong,China)

机构地区：[1]北京中医药大学中药学院,北京102488 [2]广东一方制药有限公司,广东佛山528000

出　　处：《上海中医药大学学报》2023年第5期31-38,共8页Academic Journal of Shanghai University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(82073971);北京市技术服务类横向科研项目(BUCM-2021-JS-FW-133)。

摘　　要：目的:建立不同产地野生与栽培赤芍的高效液相色谱(HPLC)指纹图谱并结合灰色关联-逼近理想解排序法(TOPSIS)分析方法评价其质量。方法:采用Agilent ZORBAX SB C18(4.6 mm×250 mm,5μm)色谱柱,以乙腈-0.1%磷酸水溶液为流动相,梯度洗脱,检测波长254 nm。结果:建立30批不同产地野生与栽培赤芍的指纹图谱,相似度为0.821~0.989;共标定了15个共有峰,指认了没食子酸、羟基芍药苷、儿茶素、芍药内酯苷、芍药苷、没食子酸乙酯、没食子酰芍药苷、苯甲酸、1,2,3,4,6-五没食子酰葡萄糖、芍药新苷、苯甲酰芍药苷11种成分;热图聚类分析显示30批不同产地野生与栽培赤芍聚为3类;灰色关联度TOPSIS分析不同产地野生与栽培赤芍的质量,综合评价河北野生赤芍质量最优;河北栽培、四川栽培赤芍品质较优;黑龙江野生、内蒙古野生、吉林栽培、山东栽培、安徽栽培赤芍质量次之;黑龙江栽培、内蒙古栽培赤芍质量较差。提示河北、四川等区域可作为赤芍的适宜栽培产地。结论:该结果可为赤芍药材的质量评价提供参考,同时也为赤芍栽培品的市场流通及种植选址提供依据。Objective:To establish high performance Liquid chromatography(HPLC)fingerprints of wild and cultivated Paeoniae Radix Rubra from different origins and to evaluate its quality by grey correlation-technique for order preference by similarity to an ideal solutiow(TOPSIS)analysis.Methods:HPLC analysis was performed on Agilent ZORBAX SB-C18 column(4.6 mm×250 mm,5μm),and the mobile phase was acetonitrile(A)-0.1%phosphate acid(B)for gradient elution.The detection wavelength was 254 nm.Results:HPLC fingerprints of 30 batches of wild and cultivated Paeoniae Radix Rubra from different origins were obtained,with similarity ranging from 0.821 to 0.989,from which 15 common peaks were calibrated and 11 components were identified,including gallic acid,hydroxypaeoniflorin,catechin,albiflorin,paeoniflorin,ethyl gallate,galloyl paeoniflorin,benzoic acid,1,2,3,4,6-pentagaloglucose,lactiflorin,benzoyl paeoniflorin.Cluster heatmap analysis showed that the 30 batches of wild and cultivated Paeoniae Radix Rubra from different origins were clustered into three categories.The quality analysis of wild and cultivated Paeoniae Radix Rubra from different origins was performed by grey correlation-TOPSIS.Overall,Hebei wild sample ranked the first and had the best quality,the quality of Hebei cultivated sample and Sichuan cultivated sample is better than that of the left samples,Heilongjiang wild sample,Nei Mongol wild sample,Jilin cultivated sample,Shandong cultivated sample and Anhui cultivated sample ranked behind,Heilongjiang cultivated sample and Nei Mongol cultivated sample had lower ranking with the poor quality.It indicated the regions such as Hebei and Sichuan can be suitable cultivation areas for Paeoniae Radix Rubra.Conclusion:This result can provide reference for quality evaluation of Paeoniae Radix Rubra and also provide the basis for market circulation and planting site selection of Paeoniae Radix Rubra.

关 键 词：赤芍 野生品 栽培品 HPLC指纹图谱 灰色关联-TOPSIS分析 质量评价 

分 类 号：R284.1[医药卫生—中药学]