基于高密度低共熔溶剂的分散液液微萃取  

High Density Deep Eutectic Solvents Based Dispersive Liquid-liquid Microextraction

作　　者：于洋 陈小雅 丁夕格 葛丹丹 王颖臻 YU Yang;CHEN Xiao-ya;DING Xi-ge;GE Dan-dan;WANG Ying-zhen(School of Chemistry and Chemical Engineering,Kunming University,Kunming 650214,China)

机构地区：[1]昆明学院化学化工学院,云南昆明650214

出　　处：《化学试剂》2023年第11期111-116,共6页Chemical Reagents

基　　金：云南省教育厅科学研究基金研究生项目(2023Y0906);云南省研究生教学案例库项目建设《高等分离工程》教学案例库建设资助项目(2022)。

摘　　要：以六氟异丙醇为氢键供体,分别以辛胺、壬胺和癸胺为氢键受体合成出一类新型高密度疏水性低共熔溶剂(DES)并作为萃取溶剂,用于涡旋辅助-分散液液微萃取-高效液相色谱法检测专利蓝V和固绿FCF。对影响萃取效率的因素进行优化,结果表明,n(辛胺)∶n(六氟异丙醇)=1∶2制备出的DES为萃取溶剂,萃取剂使用量为50μL,盐浓度为1%,pH 10,旋涡萃取时间是2 min。在最优化条件下,专利蓝V和固绿FCF在浓度6~1000 ng/mL之间有良好的线性关系;方法检出限为2.0~2.6 ng/mL,定量限为6.0~8.0 ng/mL,相对标准偏差为1.0%~10.0%。该方法简便、快速并且绿色环保,可用于水样和饮料样中的专利蓝V和固绿FCF分析。A new type of hydrophobic high density deep eutectic solvents(DESs)was prepared using hexafluoroisopropanol(HFIP,as hydrogen bond donor)and octylamine,nonylamine and decylamine(as hydrogen bond acceptor).The prepared DESs were used as extraction solvents and applied to vortex-assisted dispersive liquid-liquid microextraction of patent blue V and fast green FCF.Some parameters influencing the extraction efficiency were optimized.The obtained results show that DES prepared from octylamine and HFIP at the molar ratio of 1∶2 was used as extraction solvent,the extraction solvent volume was 50μL,the vortex time was 2 min,the salt concentration was 1%the solution was pH 10.Under the optimized conditions,a linear range of 6~1000 ng/mL with determination coefficients of<0.999 was obtained.The limits of detection were 2.0~2.6 ng/mL.The limits of quantification of the method were 6.0~8.0 ng/mL.Relative standard deviations were 1.0%~10%.This method is easy to operat,fast and environmental friendly,and can be applied to determine patent blue V and fast green FCF in environmental water and drink samples.

关 键 词：分散液液微萃取 高密度疏水性低共熔溶剂 高效液相色谱 色素 六氟异丙醇 

分 类 号：O661.1[理学—分析化学]