阿维菌素对瓜实蝇3种解毒酶系的影响  

Effects of abamectin on the three detoxification enzyme systems in Bactrocera cucurbitae(Coquillett)

作　　者：姜建军 王凤英[2] 陈黔[2] 黄立飞 曹雪梅[2] 杨朗 JIANG Jian-jun;WANG Feng-ying;CHEN Qian;HUANG Li-fei;CAO Xue-mei;YANG Lang(Guangxi Vocational University of Agriculture,Nanning,Guangxi 530007,China;Plant Protection Research Institute,Guangxi Academy of Academy of Agricultural Science/Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China,Ministry of Agriculture and Rural Affairs/Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests,Nanning,Guangxi 530007,China)

机构地区：[1]广西农业职业技术大学,广西南宁530007 [2]广西农业科学院植物保护研究所/农业农村部华南果蔬绿色防控重点实验室/广西作物病虫害生物学重点实验室,广西南宁530007

出　　处：《南方农业学报》2023年第7期1893-1902,共10页Journal of Southern Agriculture

基　　金：国家自然科学地区基金项目(31960564);广西科技重大专项(桂科AA20108002);广西农业科学院科技发展基金项目(桂农科2021JM72,桂农科2021YT068)。

摘　　要：【目的】探讨阿维菌素对瓜实蝇[Bactrocera cucurbitae(Coquillett)]解毒酶系的影响,明确瓜实蝇阿维菌素抗性与解毒酶的关系,为瓜实蝇抗药性综合治理和科学用药提供理论基础。【方法】采用饲毒法测定阿维菌素对瓜实蝇成虫敏感品系(SS)和抗阿维菌素品系(RS)的亚致死浓度(LC_(25))及致死中浓度(LC_(50));利用微孔板法测定SS品系和RS品系经阿维菌素LC_(25)、LC_(50)剂量处理12、24、48和72 h后其体内谷胱甘肽S-转移酶(Glutathione S-transferase,GSTs)、羧酸酯酶(Carboxylesterase,CarE)活性,采用ELISA试剂盒法测定细胞色素P450单加氧化酶(Cytochrome P450 monooxygenases,P450s)含量;测定比较SS品系和RS品系GSTs、CarE米氏常数(K_(m))及最大反应速率(V_(max))的变化及增效剂顺丁烯二酸二乙酯(DEM)、磷酸三苯酯(TPP)和增效醚(PBO)对阿维菌素的增效作用。【结果】与SS品系相比,RS品系体内GSTs和CarE活性均显著上升(P<0.05,下同),分别为SS品系的2.92和1.46倍,P450s含量则显著下降,为SS品系的68.0%。SS品系在LC_(25)剂量的阿维菌素处理后12、48 h及LC_(50)处理后12、24、48 h和RS品系在LC_(25)剂量处理后12 h、LC_(50)处理后72 h,其体内GSTs活性均被诱导显著增强;LC_(25)剂量处理后24 h,SS品系体内CarE活性被诱导显著增强,RS品系中LC_(25)处理后72 h和LC_(50)处理后各时间段CarE活性表现为被显著抑制;LC_(50)处理后12 h,P450s含量在SS品系中表现为显著降低,而在72 h则显著升高。与SS品系相比,RS品系GSTs的K_(m)显著降低,V_(max)显著升高,CarE的K_(m)和V_(max)变化不明显;增效剂DEM、TPP和PBO在SS品系中的增效比分别为1.03、1.04和2.08,在RS品系中的增效比分别为3.45、2.52和2.26,相对增效系数分别为0.71、0.60和0.54。【结论】GSTs和CarE解毒酶参与了瓜实蝇对阿维菌素的抗性形成,其中GSTs活性发生了量变和质变,其对底物亲和力增加和代谢速度增快,在瓜实蝇对阿维菌�【Objective】The purpose of the study was to explore the effects of abamectin on the detoxification enzyme system of Bactrocera cucurbitae(Coquillett),to clarify the relationship between abamectin resistance and detoxification enzymesin B.cucurbitae,and to provide theoretical basis for the comprehensive management of B.cucurbitae resistance and the scientific use of medication.【Method】The feeding toxicity method was used to determine the sublethal concentration(LC_(25))and lethal concentration(LC_(50))of abamectin on susceptible strain(SS)and abamectin resistant strain(RS)of adult B.cucurbitae.Microplate method was used to determine the activities of glutathione S-transferase(GSTs)and carboxylesterase(CarE)in adults of SS and RS strains and those treated with LC_(25)and LC_(50)doses for 12,24,48 and 72 h.Cytochrome P450 monooxygenases(P450s)content was determined by ELISA kit method.The changes in GSTs,Michaelis constant(K_(m))and maximal velocity(V_(max))values and synergistic effects of synergists diethyl maleate(DEM),triphenyl phosphate(TPP)and potentiometric booster ether(PBO)on abamectin were tested and analyzed in the adults of SS and RS strains.【Result】The activities of GSTs and CarE in the RS strain were significantly increased(P<0.05,the same below)compared with those in the SS strain,which were 2.92 and 1.46 times of that in the SS strain,while the P450 content was significantly decreased,which was 68.0%of that in the SS strain.After being exposed to LC_(25)for 12,48 h and LC_(50)for 12,24,48 h in SS strain and LC_(25)for 12 h,LC_(50)for 72 h in RS strain of abamectin,theactivities of GSTs were induced to be significantly enhanced.CarE activity was induced to be significantly enhanced at 24 h after LC_(25)dose treatment in the SS strain and was significantly inhibited at 72 h after LC_(25)treatment and at all times after LC_(50)treatment in the RS strain.The content of P450s was decreased at 12 h after LC_(50)treatment and was increased at 72 h after LC_(50)treatment.Compared with SS strain,th

关 键 词：瓜实蝇 阿维菌素 解毒酶 抗药性 

分 类 号：S433[农业科学—农业昆虫与害虫防治]