吸入不同浓度麻醉药对小鼠大脑核团的影响  

Effects of inhalation of general anesthetics at different concentrations on brain nuclei in mice

作　　者：赵萌萌 杨丽 蒋明 梁昕悦 施翔鹏 方芳 仓静 ZHAO Meng-meng;YANG Li;JIANG Ming;LIANG Xin-yue;SHI Xiang-peng;FANG Fang;CANG Jing(Department of Anesthesiology,Zhongshan Hospital,Fudan University,Shanghai 200032,China)

机构地区：[1]复旦大学附属中山医院麻醉科,上海200032

出　　处：《中国临床医学》2023年第5期798-805,共8页Chinese Journal of Clinical Medicine

基　　金：上海市科学技术委员会基金(22ZR1410500).

摘　　要：目的分析不同浓度、不同种类吸入性麻醉药物对小鼠大脑核团中c-Fos表达的影响,探讨核团激活(或抑制)在吸入性麻醉药诱导的意识消失中的可能作用。方法选择成年雄性C57BL/6小鼠30只,8周龄,体质量22~23 g。将小鼠随机分为5组:对照组(C组,吸入100%纯氧)、吸入0.8%异氟醚组(IL组)、吸入1.4%异氟醚组(IH组)、吸入1.3%七氟醚组(SL组)和吸入2.5%七氟醚组(SH组),每组6只。所有小鼠接受气体暴露2 h后立即进行心脏灌流和剥离取脑。采用免疫荧光染色检测小鼠全脑c-Fos表达情况,采用c-Fos和精氨酸加压素(arginine vasopressin,AVP)或c-Fos和催产素(oxytocin,OXT)共同免疫荧光染色检测小鼠视上核(supraoptic nucleus,SON)中AVP和OXT的表达情况。结果5组小鼠大脑核团中的SON、中央杏仁核(central amygdala,CeA)、终纹床核(bed nucleus of stria terminalis,BNST)、前背侧视前核(anterodorsal preoptic nucleus,ADP)、下丘脑外侧区(lateral hypothalamic area,LHA)、扣带回皮质(cingulate cortex,Cg)、次级运动皮质(secondary motor cortex,M2)的c-Fos表达数量差异有统计学意义。与C组相比,IL组、IH组、SL组和SH组SON、CeA和BNST中c-Fos阳性细胞显著增加(P<0.05),而ADP和LHA中c-Fos阳性细胞显著减少(P<0.05);IH组和SH组Cg中c-Fos阳性细胞显著减少(P<0.05);IH组M2中c-Fos阳性细胞显著减少(P<0.05)。与IL组、SL组相比,IH组和SH组SON中c-Fos阳性细胞明显增加(P<0.05)。与C组相比,IL组、IH组、SL组和SH组SON脑区AVP神经元激活百分比显著升高(P<0.0001)。结论吸入不同浓度异氟醚或七氟醚可增加小鼠大脑核团SON、CeA和BNST中c-Fos阳性细胞数量,减少小鼠大脑核团ADP、LHA、Cg和M2中c-Fos阳性细胞数量。SON脑区AVP神经元激活可能参与异氟醚或七氟醚诱导的意识消失。Objective To analyze the effects of different concentrations and different types of inhaled anesthetics on the expression of c-Fos in mouse brain nuclei,and to explore the possible role of nuclear activation(or inhibition)in the loss of consciousness induced by inhaled anesthetics.Methods A total of 30 male adult C57BL/6 mice,aged 8 weeks and weighing 22-23 g were selected.Mice were randomly divided into 5 groups:control group(group C,inhaled 100%pure oxygen),inhaled 0.8%isoflurane group(group IL),inhaled 1.4%isoflurane group(group IH),inhaled 1.3%sevoflurane group(group SL)and inhaled 2.5%sevoflurane group(group SH),6 mice in each group.All mice were transcardially perfused immediately after the gas exposure for 2 hours.Immunofluorescence staining was used to detect the c-Fos expression in the whole brain of mice.C-fos and arginine vasopressin(AVP)or c-Fos and oxytocin(OXT)immunofluorescence staining were used to detect the expression of AVP and OXT in supraoptic nucleus(SON).Results There were significant difference between 5 groups in c-Fos expression in SON,central amygdala(CeA),bed nucleus of striatum(BNST),anterodorsal preoptic nucleus(ADP),lateral hypothalamic area(LHA),cingulate cortex(Cg)and secondary motor cortex(M2).Compared with group C,the c-Fos positive cells in SON,CeA and BNST of group IL,group IH,group SL and group SH were significantly increased(P<0.05),while c-Fos positive cells in ADP and LHA were significantly decreased(P<0.05),c-Fos positive cells in Cg of group IH and group SH were significantly decreased(P<0.05)and c-Fos positive cells in M2 of group IH were significantly decreased(P<0.05).Compared with group IL and group SL,c-Fos positive cells in SON of group IH and group SH were significantly increased(P<0.05).Compared with group C,the activation percentage of AVP neurons in SON brain region of group IL,group IH,group SL and group SH was significantly increased(P<0.0001).Conclusions Inhaling different concentrations of isoflurane or sevoflurane can increase the number of c-Fos positive

关 键 词：异氟醚 七氟醚 C-FOS 大脑核团 全身麻醉 意识消失 

分 类 号：R614[医药卫生—麻醉学]