淅川乌骨鸡出壳前后胸肌的全转录组表达谱分析  

Analysis of Whole Transcriptome Expression Profile of Breast Muscles in Xichuan Black-bone Chicken before and after Hatching

作　　者：石建州[1,2] 于金冉 王彦伟 王铁军[4,5] 李娜 刘阳坤 姚伦广 SHI Jianzhou;YU Jinran;WANG Yanwei;WANG Tiejun;LI Na;LIU Yangkun;YAO Lunguang(Nanyang Normal University,Nanyang 473061,China;The Shennong Laboratory,Zhengzhou 450046,China;Zhengzhou University,Zhengzhou 450001,China;Nanyang Vocational College of Agriculture,Nanyang 473000,China;Henan Province Engineering Technology Research Center of Animal Disease Control and Prevention,Nanyang 473000,China)

机构地区：[1]南阳师范学院,河南南阳473061 [2]神农种业实验室,河南郑州450046 [3]郑州大学,河南郑州450001 [4]南阳农业职业学院,河南南阳473000 [5]河南省动物疫病防控工程技术研究中心,河南南阳473000

出　　处：《畜牧与饲料科学》2023年第5期1-7,共7页Animal Husbandry and Feed Science

基　　金：神农种业实验室一流课题项目(SN01-2022-05);河南省科技攻关项目(222102110260);河南省高等学校重点科研项目(22A180026);2022年河南省高校大学生创新创业训练计划项目(202210481048);2022年南阳市级科技计划项目(KJGG013,RKX009);南阳师范学院理工科STP项目(2023STP006);大学生实践教学活动创新项目(SPCP2023267,SPCP2023262)。

摘　　要：[目的]分析淅川乌骨鸡出壳前后胸肌生长过程中全转录组表达谱的变化。[方法]采集入孵14 d的淅川乌骨鸡鸡胚胸肌样本(记为X-14 d)和刚出壳1 d的雏鸡胸肌样本(记为X-1 d),围绕编码RNA(messenger RNA,mRNA)、非编码RNA(lncRNA、circRNA和miRNA)进行真核生物全转录组测序分析。[结果]淅川乌骨鸡胸肌生长过程中大量基因转录组水平发生显著变化,出孵1 d的雏鸡与入孵14 d的鸡胚相比(X-14 d vs X-1 d),共发现3858个差异表达mRNA,包括1054个上调基因和2804个下调基因;371个差异表达lncRNA,包括222个上调基因和149个下调基因;316个差异表达circRNA,包括148个上调基因和168个下调基因;377个差异表达miRNA,包括159个上调基因和218个下调基因;GO富集分析表明,差异表达mRNA参与多个生物学过程,如生物过程调控、刺激反应、定位、正负调节生物过程、生长过程、免疫系统过程等。对差异表达mRNA进行的KEGG通路富集分析表明,黏附连接、肌动蛋白细胞骨架的调节、氨基酸的生物合成、氧化磷酸化、碳代谢、柠檬酸循环(TCA循环)、紧密连接、间隙连接、代谢途径、焦点黏附、糖酵解/糖异生、丙酮酸代谢、黑素原生成、Notch信号通路等多个信号通路被富集。显著差异表达基因主要与淅川乌骨鸡的生长性状、肉质性状、脂质性状、黑色素生成性状、免疫系统等多个生物性状相关。[结论]mRNA、lncRNA、circRNA、miRNA作为淅川乌骨鸡基因转录组的重要组成部分,在淅川乌骨鸡的生长发育中发挥着重要作用。研究结果为解析淅川乌骨鸡胸肌生长发育过程的基因调控机制提供了参考。[Objective]This study aimed to characterize the changes in whole transcriptome expression profile of breast muscles during the growth process in Xichuan black-bone chicken before and after hatching.[Method]Breast muscle samples were collected from the Xichuan black-bone chicken embryos incubated for 14 d(marked as X-14 d)and the chicks freshly hatched for 1 d(marked as X-1 d),respectively.Eukaryotic whole transcriptome sequencing analysis were performed around coding RNA(messenger RNA,mRNA)and non-coding RNA(lncRNA,circRNA and miRNA).[Result]During the growth process of breast muscles in Xichuan black-bone chicken,the transcriptome levels of a large number of genes had significant changes.Compared with the chicken embryos incubated for 14 d,the chicks freshly hatched for 1 d(X-14 d vs X-1 d)had a total of 3858 differentially expressed mRNA,including 1054 upregulated genes and 2804 downregulated genes.There were 371 differentially expressed lncRNA,including 222 upregulated genes and 149 downregulated genes;there were 316 differentially expressed circRNA,including 148 upregulated genes and 168 downregulated genes;there were 377 differentially expressed miRNA,including 159 upregulated genes and 218 downregulated genes.GO enrichment analysis showed that differentially expressed mRNA was involved in multiple biological processes,such as biological process regulation,stimulus response,localization,positive/negative regulation of biological processes,growth processes,immune system processes,etc.The KEGG pathway enrichment analysis of differentially expressed mRNA demonstrated that multiple signaling pathways were enriched,including adhesion junction,actin cytoskeleton regulation,amino acid biosynthesis,oxidative phosphorylation,carbon metabolism,citric acid cycle(TCA cycle),tight junction,gap junction,metabolic pathway,focal adhesion,glycolysis/gluconeogenesis,pyruvic acid metabolism,melanogenesis,Notch signaling pathway,etc.The significantly differentially expressed genes were mainly related to multiple biological trai

关 键 词：淅川乌骨鸡 胸肌 全转录组表达谱 基因测序 

分 类 号：S831.2[农业科学—畜牧学]