基于自增强型互穿聚合物网络凝胶的中药缓释体系的构建与表征  

Preparation and characterization of self-reinforced interpenetrating polymer network hydrogel system for sustained release of traditional Chinese medicine

作　　者：朱日然[1] 丁波 丁胜勇[3] 黄超 张雅楠 ZHU Riran;DING Bo;DING Shengyong;HUANG Chao;ZHANG Yanan(Department of Pharmacy,Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250014,China;Yantai Institute for Food and Drug Control,Yantai 264035,China;Biomedical Sensing Engineering Technology Research Center,Shandong University,Jinan 250100,China;Department of Pharmacy,The Second Hospital,Cheeloo College of Medicine,Shandong University,Jinan 250033,China)

机构地区：[1]山东中医药大学附属医院药学部,山东济南250014 [2]烟台市食品药品检验检测中心,山东烟台264035 [3]山东大学生物医学传感工程技术研究中心,山东济南250100 [4]山东大学齐鲁医学院第二医院药学部,山东济南250033

出　　处：《药学研究》2023年第10期782-789,797,共9页Journal of Pharmaceutical Research

基　　金：山东省自然科学基金(No.ZR2022QH367、ZR2023MH249);青岛博士后项目(No.QDBSH20220201024);山东中医药大学博士后项目;山东省中医药科技项目面上项目(No.2020M012)。

摘　　要：目的构建并表征用于中药缓释的自增强互穿聚合物网络水凝胶(SR-IPNH)体系。方法基于洛沙姆网络和甘草酸(GA)网络,构建SR-IPNH体系。本研究考察了所制备凝胶的流变性、成胶性、体外可注射性、载药性能、体外释放行为及初步稳定性。结果所制备的SR-IPNH在室温下具有良好的流动性,在35.1℃下能2.5 min内快速凝胶,证明本体系具有良好的热敏成胶性能。含量分析结果表明,体系中芍药苷和甘草酸的含量分别为1.88%和0.86%。流变学考察结果显示,甘草酸能使凝胶的储能模量G′显著上升,且损耗因子tanδ显著降低,这说明体系的弹性性能得到加强,机械强度得到提升。药物体外释放研究结果显示本体系具备显著的缓释性能,揭示GA的释放受到自扩散和凝胶溶解的双重影响。此外,稳定性试验结果显示室温、密闭和黑暗环境更适合SR-IPNH的贮藏。结论本研究成功制备了SR-IPNH体系,表征结果展示出良好的机械强度和缓释性能。本工作为中医药治疗OA提供新的思路和技术支持。Objective To develop a self-reinforced interpenetrating polymer networks hydrogel(SR-IPNH)system for sustained release of traditional Chinese medicine(TCM).Methods The SR-IPNH system was constructed by crosslinking poloxamer with glycyrrhizic acid(GA).The properties of prepared hydrogel were studied,including rheological properties,gelling properties,injectability and gel-forming ability in vitro,drug loading,release behavior in vitro and preliminary stability.Results The prepared SR-IPNH with SGD showed good fluidity at room temperature and gelled successfully within 2.5 minutes at 35.1℃,which made it suitable for rapid gelation in situ at body temperature.The contents of paeoniflorin and GA were measured as 1.88%and 0.86%,respectively.In addition,low temperature(4~25℃),closed and dark environment were recommended as storage condition based on stability experiments.Conclusion A SR-IPNH system was developed successfully and showed potential for development of new dosage forms of TMC in OA treatment.

关 键 词：自增强型互穿网络水凝胶 中药 缓释体系 甘草酸 泊洛沙姆 

分 类 号：R943[医药卫生—药剂学]