METAGENOMICS COMBINED WITH HIGHTHROUGHPUT SEQUENCING REVEALS THE METHANOGENIC POTENTIAL OF FRESH CORN STRAW UNDER THERMOPHILIC AND HIGH OLR  

作　　者：Jinzhi HUANG Xiaoting YAN Zhen LIU Mengyi WANG Yangyang HU Zhenyu LI Minsong LIN Yiqing YAO 

机构地区：[1]College of Mechanical and Electronic Engineering,Northwest A&F University,Yangling 712100,China [2]School of Ecology and Environment,Northwestern Polytechnical University,Xi’an 710072,China [3]College of Food Science and Engineering,Northwest A&F University,Yangling 712100,China [4]Chinese Academy for Environmental Planning,Ministry of Ecology and Environment of the People’s Republic of China,Beijing 100012,China [5]Northwest Research Center of Rural Renewable Energy,Exploitation and Utilization of Ministry of Agriculture,Northwest A&F University,Yangling 712100,China

出　　处：《Frontiers of Agricultural Science and Engineering》2023年第3期403-423,共21页农业科学与工程前沿（英文版）

基　　金：supported by the Shaanxi Youth Thousand Talents Project(A279021901);the Scientific and Technological Activities for Overseas Researchers in Shaanxi Province(20200002);the Chinese Universities Scientific Fund(2452021112);the Key Research and Development Project of Shaanxi Province(2020NY-114);the Double first-class construction project funded by Northwest A&F University,Northwest A&F University Young Talent Project(Z111021902);the USA Energy Foundation(G-2206-33957).

摘　　要：Dry corn straw(DCS)is usually used in anaerobic digestion(AD),but fresh corn straw(FCS)has been given less consideration.In this study,the thermophilic AD of single-substrate(FCS and DCS)and co-digestion(straw with cattle manure)were investigated.The results show that when FCS was used as the single-substrate for AD,the methane production was 144 mL·g^(−1)·VS^(−1),which was 7.5%and 19.6%higher than that of single DCS and FCS with cattle manure,respectively.In addition,the structure of FCS was loose and coarse,which was easier to be degraded than DCS.At the hydrolysis and acidification stages,Clostridium_sensu_stricto_1,Clostridium_sensu_stricto_7 and Sporosarcina promoted the decomposition of organic matter,leading to volatile fatty acids(VFAs)accumulation.Methanosarcina(54.4%)activated multifunctional methanogenic pathways to avoid the VFAs inhibition,which was important at the CH_(4) production stage.The main pathway was hydrogenotrophic methanogenesis,with genes encoding formylmethanofuran dehydrogenase(K00200-K00203)and tetrahydromethanopterin Smethyltransferase(K00577-K00584).Methanosarcina also activated acetotrophic and methylotrophic methanogenesis pathways,with genes encoding acetyl phosphate(K13788)and methyl-coenzyme M reductase(K04480,K14080 and K14081),respectively.In the co-digestion,the methanogenic potential of FCS was also confirmed.This provides a scientific basis for regulating AD of crop straw.

关 键 词：fresh corn straw high solid anaerobic digestion METAGENOMICS microbial communities THERMOPHILIC 

分 类 号：TP2[自动化与计算机技术—检测技术与自动化装置]