Condensation of STM is critical for shoot meristem maintenance and salt tolerance in Arabidopsis  

作　　者：Xiuwei Cao Qingwei Du Yahe Guo Ying Wang Yuling Jiao 

机构地区：[1]State Key Laboratory of Plant Genomics and National Center for Plant Gene Research(Beijing),Institute of Genetics and Developmental Biology,The Innovative Academy of Seed Design,Chinese Academy of Sciences,Beijing 100101,China [2]College of Life Sciences,University of Chinese Academy of Sciences,Beijing 100049,China [3]Beijing Agro-Biotechnology Research Center,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China [4]State Key Laboratory of Protein and Plant Gene Research,School of Life Sciences,Peking University,Beijing 100871,China [5]Peking-Tsinghua Center for Life Sciences,Center for Quantitative Biology,Academy for Advanced Interdisciplinary Studies,Peking University,Beijing 100871,China [6]Peking University Institute of Advanced Agricultural Sciences,Shandong Laboratory of Advanced Agricultural Sciences in Weifang,Weifang,Shandong 261325,China [7]College of Advanced Agricultural Sciences,University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Molecular Plant》2023年第9期1445-1459,共15页分子植物（英文版）

基　　金：the Natural Science Foundation of China(grants 31825002 and 32230010 to Y.J.,and 32270345 to Y.W.);X.C.is a fellow of the China Postdoctoral Science Foundation(2020M670515);the Newton Advanced Fellowship of the Royal Society(NAF\R1\180125).

摘　　要：The shoot meristem generates the entire shoot system and is precisely maintained throughout the life cycle under various environmental challenges.In this study,we identified a prion-like domain(PrD)in the key shoot meristem regulator SHOOT MERISTEMLESS(STM),which distinguishes STM from other related KNOX1 proteins.We demonstrated that PrD stimulates STM to form nuclear condensates,which are required for maintaining the shoot meristem.STM nuclear condensate formation is stabilized by selected PrD-containing STM-interacting BELL proteins in vitro and in vivo.Moreover,condensation of STM promotes its interaction with the Mediator complex subunit MED8 and thereby enhances its transcriptional activity.Thus,condensate formation emerges as a novel regulatory mechanism of shoot meristem functions.Furthermore,we found that the formation of STM condensates is enhanced upon salt stress,which allows enhanced salt tolerance and increased shoot branching.Our findings highlight that the transcription factor partitioning plays an important role in cell fate determination and might also act as a tunable environmental acclimation mechanism.

关 键 词：intrinsically disordered proteins KNOX1 shoot apical meristem biomolecular condensate salt stress axillary meristem 

分 类 号：O57[理学—粒子物理与原子核物理]