Balanced nitrogen–iron sufficiency boosts grain yield and nitrogen use efficiency by promoting tillering  

作　　者：Ying Song Guang-Yu Wan Jing-Xian Wang Zi-Sheng Zhang Jin-Qiu Xia Liang-Qi Sun Jie Lu Chuan-Xi Ma Lin-Hui Yu Cheng-Bin Xiang Jie Wu 

机构地区：[1]Division of Life Sciences and Medicine,Division of Molecular&Cell Biophysics,Hefei National Science Center for Interdisciplinary Sciences at the Microscale,MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics,University of Science and Technology of China,The Innovation Academy of Seed Design,Chinese Academy of Sciences,Hefei,Anhui Province 230027,China [2]School of Agronomy,Anhui Agricultural University,Hefei,Anhui Province 230036,China [3]State Key Laboratory of Crop Stress Biology for Arid Areas and Institute of Future Agriculture,Northwest A&F University,Yangling,Shaanxi 712100,China

出　　处：《Molecular Plant》2023年第10期1661-1677,共17页分子植物（英文版）

基　　金：supported by grants from the National Natural Science Foundation of China(grant no.32321001 to C.B.X.and 32100208 to J.W.);the Strategic Priority Research Program of the Chinese Academy of Sciences(grant no.XDA24010303);the Anhui Provincial Natural Science Foundation(grant no.2108085QC103);the Fundamental Research Funds for the Central Universities(grant no.WK9100000023).

摘　　要：Crop yield plays a critical role in global food security.For optimal plant growth and maximal crop yields,nutrients must be balanced.However,the potential significance of balanced nitrogen-iron(N-Fe)for improving crop yield and nitrogen use efficiency(NUE)has not previously been addressed.Here,we show that balanced N-Fe sufficiency significantly increases tiller number and boosts yield and NUE in rice and wheat.NIN-like protein 4(OsNLP4)plays a pivotal role in maintaining the N-Fe balance by coordinately regulating the expression of multiple genes involved in N and Fe metabolism and signaling.OsNLP4 also suppresses OsD3 expression and strigolactone(SL)signaling,thereby promoting tillering.Balanced N-Fe sufficiency promotes the nuclear localization of OsNLP4 by reducing H_(2)O_(2) levels,reinforcing the functions of OsNLP4.Interestingly,we found that OsNLP4 upregulates the expression of a set of H2O2-scavenging genes to promote its own accumulation in the nucleus.Furthermore,we demonstrated that foliar spraying of balanced N-Fe fertilizer at the tillering stage can effectively increase tiller number,yield,and NUE of both rice and wheat in the field.Collectively,these findings reveal the previously unrecognized effects of N-Fe balance on grain yield and NUE as well as the molecular mechanism by which the OsNLP4-OsD3 module integrates N-Fe nutrient signals to downregulate SL signaling and thereby promote rice tillering.Our study sheds light on how N-Fe nutrient signals modulate rice tillering and provide potential innovative approaches that improve crop yield with reduced N fertilizer input for benefitting sustainable agriculture worldwide.

关 键 词：nitrogen-iron balance tller NUE grain yield OsNLP4 rice 

分 类 号：F326.1[经济管理—产业经济]