黄姜花茎黄酮提取工艺优化及抗氧化活性研究  

Study on optimization of extraction process and antioxidant activity of flavonoids from Hedychium flavum Roxb stem

作　　者：杨婷 孟攀 徐霖 田野 林长松 YANG Ting;MENG Pan;XU Lin;TIAN Ye;LIN Chang-song

机构地区：[1]贵州理工学院食品药品制造工程学院,贵州贵阳550005 [2]贵阳学院生物与环境工程学院,贵州贵阳550005

出　　处：《饲料研究》2023年第20期114-119,共6页Feed Research

基　　金：贵州理工学院高层次人才科研启动经费项目资助(项目编号XJGC20190922);贵阳市科技局-贵阳学院专项资金(项目编号:GYU-KYZ(2019—2020)PT16-03);2021年贵州省大学生创新创业训练项目(项目编号:S202114440044)。

摘　　要：试验旨在优化黄姜花茎黄酮提取工艺。采用超声辅助乙醇提取黄姜花茎黄酮,在单因素试验的基础上,以黄酮提取量为响应值,对影响黄姜花茎黄酮提取量的4个因素进行Box-Behnken设计与优化,分析提取过程中乙醇浓度、液料比、温度和提取时间对黄姜花茎黄酮提取量的影响,测定黄姜花茎黄酮提取物的体外抗氧化性。结果表明,黄姜花茎黄酮最佳提取工艺条件为液料比15 mL/g、温度63.0℃、提取时间32.5 min、乙醇浓度90%,在此最佳条件下黄姜花茎黄酮提取量为47.97 mg/g,与预测值基本一致,表明此优化方法合理可靠。体外抗氧化试验显示,黄姜花茎黄酮提取物具有较强的DPPH自由基、ABTS自由基清除能力。研究表明,黄姜花茎黄酮提取物具有较好的抗氧化活性。The study was to optimize the extraction process of flavonoids from of Hedychium flavum Roxb stem.Ultrasonic-assisted ethanol extraction was used to extract flavonoids from Hedychium flavum Roxb stem.Based on the single factor experiment,the extraction amount of flavonoids was used as the response value.Box-Behnken design and optimization were carried out on the four factors affecting the extraction amount of flavonoids from Hedychium flavum Roxb stem,analyzed the effect of ethanol concentration,liquid to solid ratio,temperature and extraction time on the extraction amount of flavonoids from Hedychium flavum Roxb stem,determined the in vitro antioxidant activity of flavonoid extracts from Hedychium flavum Roxb stem.The results showed that the optimum extraction conditions were liquid to solid of 15 mL/g,the temperature of 63.0℃,the extraction time of 32.5 min,and the ethanol concentration of 90%.Under the optimum conditions,the extraction amount of flavonoids from Hedychium flavum Roxb stem was 47.97 mg/g,which was basically consistent with the predicted value,indicating that the optimization method was reasonable and reliable.The in vitro antioxidant test showed that the flavonoid extract of the rhizome had strong DPPH and ABTS scavenging ability.The study indicates that the flavonoid extract of the rhizome had good antioxidant activity.

关 键 词：黄姜花 黄酮 抗氧化 响应面优化 

分 类 号：S816[农业科学—饲料科学]