肠润方对慢传输型便秘小鼠肠道传输功能的影响及机制研究  

Effects and Mechanism of Changrun Decoction on Intestinal Transport Function in Mice with Slow Transit Constipation

作　　者：洪燕秋 林金荣[1,2] 魏晓玲 肖秋平 HONG Yanqiu;LIN Jinrong;WEI Xiaoling;XIAO Qiuping(Xiamen Hospital of Traditional Chinese Medicine,Fujian University of Traditional Chinese Medicine,Xiamen,Fujian,361009,China;Xiamen Key Laboratory of Pelvic Floor Dynamics,Xiamen,Fujian,361009,China)

机构地区：[1]福建中医药大学附属厦门中医院,福建厦门361009 [2]厦门市盆底动力学重点实验室,福建厦门361009

出　　处：《福建中医药》2023年第10期15-19,共5页Fujian Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金青年科学基金项目(82305255);厦门市自然科学基金项目联合项目(3502Z20227364);厦门市扶持中医药发展专项资助项目(XWZY-2023-0614)。

摘　　要：目的探讨肠润方对慢传输型便秘(STC)小鼠肠道传输功能的影响和作用机制。方法将60只SPF级C57BL/6J小鼠随机分为正常组15只和造模组45只。正常组不予造模,造模组按体质量12.5 mg/(kg·d)灌胃复方地芬诺酯混悬液构建STC小鼠模型。造模2周后,2组各随机选取5只小鼠,造模组与正常组比较,首粒黑便排出时间延长、粪便含水率降低、墨汁推进率减低则判定造模成功。造模成功后造模组随机分为模型组,高、中、低剂量组各10只。高、中、低剂量组分别按体质量24.7、12.35、6.175 g/(kg·d)灌胃肠润方水煎液,模型组、正常组按体质量12.5 mL/(kg·d)灌胃生理盐水,连续干预2周。2周后观察5组小鼠首粒黑便排出时间、粪便含水率、墨汁推进率,Western blot检测结肠组织蛋白基因产物9.5(PGP9.5)、受体酪氨酸激酶(c-kit)、干细胞因子(SCF)蛋白表达量,qPCR检测结肠组织PGP9.5、c-kit、SCF mRNA相对表达水平。结果与正常组比较,模型组首粒黑便排出时间延长,粪便含水率、墨汁推进率均降低(P<0.05),PGP9.5、c-kit、SCF蛋白表达量和mRNA相对表达水平均降低(P<0.05);与模型组比较,高剂量组、中剂量组首粒黑便排出时间均缩短,粪便含水率、墨汁推进率均升高(P<0.05),低剂量组首粒黑便排出时间缩短,墨汁推进率升高(P<0.05);与低剂量组比较,高剂量组粪便含水率升高,高剂量组、中剂量组墨汁推进率均升高(P<0.05);与模型组比较,高剂量组PGP9.5、c-kit、SCF蛋白表达量均升高(P<0.05),中剂量组、低剂量组仅c-kit、SCF蛋白表达量升高(P<0.05);与低剂量组比较,高剂量组PGP9.5、c-kit蛋白表达量均升高(P<0.05);与中剂量组比较,高剂量组SCF、c-kit蛋白表达量均升高(P<0.05);与模型组比较,高剂量组PGP9.5、c-kit、SCF mRNA相对表达水平均升高(P<0.05);中剂量组仅c-kit、SCF mRNA相对表达水平升高(P<0.05),低剂量组仅c-kit mRNA相对表达水平升高(P<Objective:To explore the effects and mechanism of Changrun Decoction on the intestinal transport function of slow transit constipation(STC)mice.Methods:A total of sixty SPF-grade C57BL/6J mice were randomly divided into blank group of 15 mice and a modelling group of 45 mice.The blank group was not modeled,and the modeling group was gavaged with compound diphenoxylate suspension at 12.5 mg/(kg·d).After 2 weeks of modeling,5 mice were randomly selected from each of the 2 groups.Compared with the blank group,the first black stool defecation prolonged,fecal water content reduced,and ink advancement rate decreased in the model group,which meant the modeling was considered to be successful.After successful modeling,the modeling group was randomly divided into the model group and Changrun Decoction high,medium and low dose groups,with 10 mice in each group.The high,medium and low dose groups were given 24.7,12.35 and 6.175 g/(kg·d)of Changrun Decoction by gavage respectively,while the model and blank groups were given equal doses of saline by gavage for 2 consecutive weeks.After 2 weeks,the first black stool defecation,fecal water content,and ink advancement rate were observed in 5 groups of mice.Western blot detected the protein gene product 9.5(PGP 9.5),receptor tyrosine kinase(c-kit),and stem cell factor(SCF)protein expression in colon tissues,and qPCR detected the relative expression of PGP 9.5,c-kit,SCF mRNA in colon tissues.Results:Compared with the blank group,the modelling group had a longer time to the first black stool,a lower fecal water content and ink advancement rate(P<0.05),and a lower PGP 9.5,c-kit,SCF protein expression and mRNA relative expression level(P<0.05);compared with the model group,the high dose and the middle dose groups had a shorter time to the first black stool,a higher fecal water content and ink advancement rate(P<0.05),the low-dose group had a shorter time to first black stool time and higher ink advancement rate(P<0.05);compared with the low-dose group,fecal water content elevated i

关 键 词：慢传输型便秘 肠润方 PGP9.5 C-KIT SCF 

分 类 号：R285.5[医药卫生—中药学]