SSR荧光标记毛细管电泳分析16个博落回种质资源的遗传多样性  

Genetic Diversity Analysis of 16 Macleaya cordata Germplasm Resources by Capillary Electrophoresis with SSR Fluorescent Markers

作　　者：牛雨晴 朱育菁[1] 郑梅霞[1] 朱雁鸣 陈宏[1] 苏海兰[1] NIU Yu-qing;ZHU Yu-jing;ZHENG Mei-xia;ZHU Yan-ming;CHEN Hong;SU Hai-lan(Institute of Crop Sciences,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350003,China)

机构地区：[1]福建省农业科学院作物研究所,福建福州350003

出　　处：《福建农业科技》2023年第7期10-15,共6页Fujian Agricultural Science and Technology

基　　金：福建省科技计划公益类项目(2020R1034008);福建省农业科学院科技创新团队建设项目(2022KF21)。

摘　　要：为分析我国13个省份博落回种质资源的遗传多样性,明确其亲缘关系和遗传差异。利用SSR荧光标记毛细管电泳技术对16份博落回种质资源进行遗传多样性分析。结果表明:16个博落回种质资源多态信息含量(PIC)值的变化范围为0.375~0.687,遗传相似系数范围为0.0714~0.7142。聚类分析结果显示,在相似系数0.3处可将16份博落回种质资源分为3个大类群,第Ⅰ大类有7份种质资源,第Ⅱ大类有1份种质资源,第Ⅲ大类有8份种质资源。此外,构建了16份博落回种质资源的DNA指纹图谱数据库,开发了相对应的指纹图谱。研究结果可为博落回种质资源的分类鉴定、遗传多样性分析和品种选育提供可用的分子标记以及参考依据。In order to analyze the genetic diversity of Macleaya cordata germplasm resources in 13 provinces of China,the genetic relationship and genetic differentiation were clarified.The genetic diversity of 16 germplasm resources of Macleaya cordata was analyzed by capillary electrophoresis with SSR fluorescent markers.The polymorphic information content(PIC)values of 16 Macleaya cordata germplasm resources ranged from 0.375 to 0.687,and the genetic similarity coefficient ranged from 0.0714 to 0.7142.The cluster analysis results showed that:the 16 germplasm resources of Macleaya cordata could be divided into three major categories at the similarity coefficient of 0.3.There were 7 germplasm resources in the first major category,1 germplasm resource in the second major category and 8 germplasm resources in the third major category.In addition,the DNA fingerprint database of 16 Macleaya cordata germplasm resources was constructed,and the corresponding fingerprints were developed.The results could provide available molecular markers and reference for the classification and identification,genetic diversity analysis and variety breeding of Macleaya cordata germplasm resources.

关 键 词：博落回 SSR 分子标记 遗传多样性 

分 类 号：S567.239[农业科学—中草药栽培]