pH响应性磁性介孔纳米载药系统的制备  

Preparation of pH responsive magnetic mesoporous nanoparticle drug loading system

作　　者：刘慧慧 史笑飞 王倩男 刘锦博 张静[1] LIU Huihui;SHI Xiaofei;WANG Qiannan;LIU Jinbo;ZHANG Jing(College of Chemical Engineering,Zhengzhou University,Zhengzhou 450001,Henan,China)

机构地区：[1]郑州大学化工学院,河南郑州450001

出　　处：《化工进展》2023年第10期5390-5398,共9页Chemical Industry and Engineering Progress

摘　　要：研究开发了一种基于磁性介孔二氧化硅和聚多巴胺(PDA)混合体的智能给药平台,可用于pH响应性给药。通过共沉淀法制备Fe_(3)O_(4),以十六烷基三甲基溴化铵(CTAB)为模板,硅酸四乙酯(TEOS)为硅源,通过Stober法获得了磁性纳米材料Fe_(3)O_(4)@mSiO_(2)。负载抗癌药物阿霉素(DOX)后,通过碱性条件下多巴胺的氧化自聚合,在其表面形成pH敏感的PDA涂层,得到DOX/Fe_(3)O_(4)@mSiO_(2)@PDA磁性纳米载药系统。通过透射电子显微镜、X射线衍射、傅里叶红外光谱仪、比表面积测试和振动样品磁强计等技术手段对材料进行表征。结果表明Fe_(3)O_(4)@mSiO_(2)的形貌为球形,拥有良好介孔结构和高达619.16m^(2)/g的比表面积;VSM表明载体Fe_(3)O_(4)@mSiO_(2)@PDA具有良好的磁性。当载药过程中DOX浓度为0.5mg/mL、温度为37℃,反应36h后,载药率为51.69%,包封率为82.70%。释药曲线表明纳米载药系统具有pH响应性和药物缓释的特点;噻唑蓝比色法(MTT法)细胞毒性实验表明Fe_(3)O_(4)@mSiO_(2)@PDA生物相容性良好,DOX/Fe_(3)O_(4)@mSiO_(2)@PDA有良好的抗肿瘤效果。该纳米载药系统在药物靶向递送中具有潜在的应用前景。We developed an intelligent drug delivery platform based on the mixture of magnetic mesoporous silica and polydopamine(PDA),which can be used for pH responsive drug delivery.Magnetic cores Fe_(3)O_(4)was prepared by co-precipitation method.The magnetic nanomaterial Fe_(3)O_(4)@mSiO_(2)was obtained by the Stober method using cetyltrimethylammonium bromide(CTAB)as a template and tetraethyl silicate(TEOS)as a silicon source.Anticancer drug doxorubicin(DOX)was used as the model drug to be encapsulated into Fe_(3)O_(4)@mSiO_(2).The surface of the carrier was modified with pH-sensitive PDA coating by oxidation self-polymerization of dopamine at alkaline pH to obtain the magnetic nano drug loading system DOX/Fe_(3)O_(4)@mSiO_(2)@PDA.The materials were characterized by Transmission electron microscope,X-ray diffraction,Fourier Transform infrared spectrometer,Brunauer Emmett Teller and vibration sample magnetometer.We found that Fe_(3)O_(4)@mSiO_(2)was spherical with good mesoporous structure and a specific surface area of up to 619.16m2/g.VSM indicated that the carrier Fe_(3)O_(4)@mSiO_(2)@PDA had good magnetic properties.During the drug loading process,when DOX concentration was 0.5mg/mL,temperature was 37℃,and reaction time was 36h,the drug loading rate and encapsulation rate were up to 51.69%and 82.70%,respectively.The drug release curve showed that the nano drug delivery system had the characteristics of pH responsiveness and slow drug release.Thiazole blue colorimetric assay(MTT)cytotoxicity showed that Fe_(3)O_(4)@mSiO_(2)@PDA had good biocompatibility,and DOX/Fe_(3)O_(4)@mSiO_(2)@PDA had obvious inhibitory effect on cancer cells.The nano drug delivery system has a potential application prospect in targeted drug delivery.

关 键 词：四氧化三铁 磁性纳米材料 介孔 PH响应性 药物递送 阿霉素 

分 类 号：O614.881[理学—无机化学]