冷原子吸收测定尿中汞的方法优化与结果表述  

Method optimization and result presentation for the determination of mercury in urine by cold atomic absorptionmethod

作　　者：张晓梅 荣利 孟祥娟 刘晓东 ZHANG Xiaomei;RONG Li;MENG Xiangjuan;LIU Xiaodong(Central Laboratory,Beijing Prevention and Treatment Hospital of Occupational Disease for Chemical Industry,Beijing,100093,China)

机构地区：[1]北京市化工职业病防治院中心实验室,北京100093

出　　处：《职业与健康》2023年第18期2486-2489,共4页Occupation and Health

摘　　要：目的 优化冷原子吸收法测定尿汞含量,规范测定结果表述。方法 尿样以硫酸高锰酸钾水浴消解,在线加入氯化亚锡,将汞离子还原为元素态汞,以氩气载入光吸收池,吸光度和汞含量在一定范围内呈正比,外标法定量。结果 本方法对尿汞的测定在0~5μg/L的范围内线性情况良好,相关系数大于0.999 9,方法的检出限和定量限分别为0.007和0.025μg/L。尿样经低、中、高浓度加标,批内精密度为0.31%~0.89%,批间精密度为1.40%~2.82%,样品的平均加标回收率为88.68%~90.15%。对高、低2个水平质控样品的测定结果均在标准证书参考范围内,尿汞检测结果的单位应转换为μmol/mol肌酐(Creatinine,Cr)或μg/g Cr。结论 本文优化了尿中汞的冷原子吸收分析方法,样品经80℃水浴消解90 min后在线加入氯化亚锡,反应密闭,安全高效,方法的准确度和精密度均符合标准要求,可用于普通人群和职业接触人群尿中汞的监测评估。尿汞检测结果的表述应符合标准要求,同时对结果的判断应考虑其肌酐测定方式和样品来源。Objective To optimize the determination of urinary mercury content by cold atomic absorption methodand standardize the expression of the determination results.Methods The urine sample was digested with potassium permanganate sulfate water bath,and stannous chloride was added online to reduce the mercury ions to elemental mercury,and then loaded into the light absorption cell with argon.The absorbance is proportional to the mercury content within a certain range and can be quantified by external standard method.Results The method for the determination of mercury in urine showed good linearity in the range of 0-5μg/L,and the correlation coefficient was greater than 0.9999.The detection limit and quantification limit of the method was 0.007 and 0.025μg/L,respectively.The intra-assay precision of urine samples after being spiked with low,medium and high concentrations was 0.31%-0.89%,and the inter-assay precision was 1.40%-2.82%.The average recovery rate was 88.68%-90.15%.The determination results for the high and low levels of quality control samples are all within the reference range of the standard certificate.The unit of urine mercury test results should be converted toμmol/mol Cr(Creatinine)orμg/gCr.Conclusions In this paper,the cold atomic absorption analysis method for mercury in urine is optimized.After the sample is digested in a water bath at 80℃for 90 minutes,stannous chloride is added online,the reaction is airtight,safe and efficient.The accuracy and precision of the method meet the standard requirements and can be used for the monitoring and evaluation of urinary mercury in the general population and occupational exposure population.The presentation of the urine mercury test results should meet the standard requirements,and the creatinine measurement method and sample source should be considered in the judgment of the results.

关 键 词：尿汞 冷原子吸收 在线氯化亚锡 结果表述 

分 类 号：R135[医药卫生—劳动卫生] R446.12[医药卫生—公共卫生与预防医学]