Spatially resolved transcriptomics: a comprehensive review of their technological advances, applications, and challenges  

作　　者：Mengnan Cheng Yujia Jiang Jiangshan Xu Alexios-Fotios AMentis Shuai Wang Huiwen Zheng Sunil Kumar Sahu Longqi Liu Xun Xu 

机构地区：[1]BGI-Hangzhou,Hangzhou,Zhejiang 310012,China [2]BGI-Shenzhen,Shenzhen,Guangdong 518103,China [3]College of Life Sciences,University of Chinese Academy of Sciences,Beijing 100049,China [4]State Key Laboratory of Agricultural Genomics,BGI-Shenzhen,Shenzhen,Guangdong 518083,China [5]Guangdong Provincial Key Laboratory of Genome Read and Write,Shenzhen,Guangdong 518120,China

出　　处：《Journal of Genetics and Genomics》2023年第9期625-640,共16页遗传学报（英文版）

基　　金：supported by the Shenzhen Key Laboratory of Single-Cell Omics(ZDSYS20190902093613831);the Guangdong Provincial Key Laboratory of Genome Read and Write(2017B030301011);Longqi Liu was supported by the National Natural Science Foundation of China(31900466).

摘　　要：The ability to explore life kingdoms is largely driven by innovations and breakthroughs in technology,from the invention of the microscope 350 years ago to the recent emergence of single-cell sequencing,by which the scientific community has been able to visualize life at an unprecedented resolution.Most recently,the Spatially Resolved Transcriptomics(SRT)technologies have filled the gap in probing the spatial or even three-dimensional organization of the molecular foundation behind the molecular mysteries of life,including the origin of different cellular populations developed from totipotent cells and human diseases.In this review,we introduce recent progresses and challenges on SRT from the perspectives of technologies and bioinformatic tools,as well as the representative SRT applications.With the currently fast-moving progress of the SRT technologies and promising results from early adopted research projects,we can foresee the bright future of such new tools in understanding life at the most profound analytical level.

关 键 词：Spatially resolved transcriptomics Bioinformatic tools Spatial single-cell Cell communications Tissue dissection Three-dimensional organ 

分 类 号：X70[环境科学与工程—环境工程]