Single-cell RNA-Seq reveals transcriptional regulatory networks directing the development of mouse maxillary prominence  

作　　者：Jian Sun Yijun Lin Nayoung Ha Jianfei Zhang Weiqi Wang Xudong Wang Qian Bian 

机构地区：[1]Department of Oral and Cranio-Maxillofacial Surgery,Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200011,China [2]National Clinical Research Center for Oral Diseases,Shanghai Key Laboratory of Stomatology,Shanghai Research Institute of Stomatology,Shanghai 200011,China [3]Shanghai Institute of Precision Medicine,Shanghai 200125,China [4]Shanghai Key Laboratory of Reproductive Medicine,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China

出　　处：《Journal of Genetics and Genomics》2023年第9期676-687,共12页遗传学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(82071096 to X.W,31970585,32170544,and 31801056 to Q.B.);the National Key Research and Development Program of China(2017YFC1001800 to X.W.,2018YFC1004703 to Q.B),the Fundamental research program funding of Ninth People’s Hospital affiliated to Shanghai Jiao Tong University School of Medicine(JYZZ179 to J.S.);the Innovative research team of high-level local universities in Shanghai(SHSMU-ZLCX20211700);the SHIPM-pi fund No.JY201803 from Shanghai Institute of Precision Medicine,Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine.

摘　　要：During vertebrate embryonic development,neural crest-derived ectomesenchyme within the maxillary prominences undergoes precisely coordinated proliferation and differentiation to give rise to diverse craniofacial structures,such as tooth and palate.However,the transcriptional regulatory networks underpinning such an intricate process have not been fully elucidated.Here,we perform single-cell RNA-Seq to comprehensively characterize the transcriptional dynamics during mouse maxillary development from embryonic day(E)10.5eE14.5.Our single-cell transcriptome atlas of~28,000 cells uncovers mesenchymal cell populations representing distinct differentiating states and reveals their developmental trajectory,suggesting that the segregation of dental from the palatal mesenchyme occurs at E11.5.Moreover,we identify a series of key transcription factors(TFs)associated with mesenchymal fate transitions and deduce the gene regulatory networks directed by these TFs.Collectively,our study provides important resources and insights for achieving a systems-level understanding of craniofacial morphogenesis and abnormality.

关 键 词：Craniofacial development Single-cell RNA-Seq Maxillary prominences Gene regulatory network Transcription factor 

分 类 号：TN9[电子电信—信息与通信工程]