A biologically stable, self-catalytic DNAzyme machine encapsulated by metal-phenolic nanoshells for multiple microRNA imaging  

作　　者：Xin Jin Qin Wang Jiezhou Pan Jin Wang Yunxiang He Jiaojiao Shang Mei Chen Xianglian He Yaoyao Zhang Bo Wang Yajie Wang Guidong Gong Junling Guo 

机构地区：[1]BMI Center for Biomass Materials and Nanointerfaces,College of Biomass Science and Engineering,Sichuan University,Chengdu 610065,China [2]School of Pharmacy,Southwest Minzu University,Chengdu 610225,China [3]Department of Chemical and Biological Engineering,Bioproducts Institute,University of British Columbia,Vancouver BC V6T 1Z4,Canada [4]State Key Laboratory of Polymer Materials Engineering,Sichuan University,Chengdu 610065,China [5]National Engineering Laboratory for Clean Technology of Leather Manufacture,Sichuan University,Chengdu 610065,China [6]Department of Obstetrics and Gynecology,Key Laboratory of Birth Defects and Related of Women and Children of Ministry of Education,The Reproductive Medical Center,West China Second University Hospital,Sichuan University,Chengdu 610041,China [7]CAS Key Laboratory of Quantitative Engineering Biology,Shenzhen Institute of Synthetic Biology,Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences,Shenzhen 518055,China [8]School of Engineering,Westlake University,Hangzhou 310034,China

出　　处：《Chinese Chemical Letters》2023年第10期197-203,共7页中国化学快报（英文版）

基　　金：supported by National Talents Program,Double First Class University Plan of Sichuan University,State Key Laboratory of Polymer Materials Engineering(No.sklpme 2020-0301);Natural Science Foundation of Sichuan Province(Nos.2022NSFSC1735,2023NSFSC1097);Fundamental Research Funds for the Central Universities(No.ZYN2022094);National Natural Science Foundation of China(Nos.22178233,22208228);China Postdoctoral Science Foundation(No.2020TQ0209);Fundamental Research Funds for the Central Universities(No.YJ201959);Science and Technology Support Program of Sichuan Province(No.2021YJ0414);Project of Chengdu Science and Technology Bureau(No.2021YF05-02110-SN)。

摘　　要：DNAzyme machines play critical roles in the fields of cell imaging, disease diagnosis, and cancer therapy. However, the applications of DNAzyme machines are limited by the nucleases-induced degradation,non-specific binding of proteins, and insufficient provision of cofactors. Herein, protected DNAzyme machines with different cofactor designs(referred to as Pro Ds) were nanoengineered by the construction of multifunctional metal-phenolic nanoshells to deactivate the interferential proteins, including nucleases and non-specific binding proteins. Moreover, the nanoshells not only facilitate the cellular internalization of Pro Ds but provide specific metal ions acting as cofactors of the designed DNAzymes. Cellular imaging results demonstrated that Pro Ds could effectively and simultaneously monitor multiple tumor-related micro RNAs in living cells. This facile and rapid strategy that encapsulates DNAzyme machines into the protective metal-phenolic nanoshells is anticipated to extend to a wide range of functional nucleic acidsbased biomedical applications.

关 键 词：DNAzyme machine Metal-phenolic nanoshells Interference protection Cofactors self-supply Cell imaging 

分 类 号：Q503[生物学—生物化学] TB383.1[一般工业技术—材料科学与工程]