β-Cyclodextrin derived full-spectrum fluorescent carbon dots: The formation process investigation and biological applications  

作　　者：Peide Zhu Wenjing Li Yuqi Zhang Qiang Sun Yi Lin Ailin Qiu Xinyi Chen Yujin Zhou Guangfu Wu Yifan Li Zhiqiang Yu Quan Xu Dixian Luo Lulu Cai 

机构地区：[1]State Key Laboratory of Heavy Oil Processing,China University of Petroleum-Beijing,Beijing 102249,China [2]Department of Laboratory Medicine,Huazhong University of Science and Technology Union Shenzhen Hospital(Nanshan Hospital),Shenzhen 518052,China [3]Personalized Drug Therapy Key Laboratory of Sichuan Province,Department of Pharmacy,Sichuan Provincial People’s Hospital,University of Electronic Science and Technology of China,Chengdu 611731,China [4]Department of Laboratory Medicine,Dongguan Institute of Clinical Cancer Research,Affiliated Dongguan Hospital,Southern Medical University,Dongguan 523018,China

出　　处：《Chinese Chemical Letters》2023年第10期215-222,共8页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.U2230123);the Science Foundation of the Science and Technology Department of Sichuan Province(No.22ZYZYTS0159);Science Foundation of China University of Petroleum(Nos.2462019QNXZ02,2462019BJRC007);Science Foundation of China University of Petroleum(East China)(No.2462020YXZZ018);Science and Technology Innovation Commission of Shenzhen(No.JSGG20210802153410031);Science and Technology Project of Nanshan District(No.NS_(2)021016);the Scientific Research Startup Fund for Discipline Leader of Huazhong University of Science and Technology Union Shenzhen Hospital(Nanshan Hospital)(No.YN2021002)。

摘　　要：Carbon dots(CDs), a new building unit, have been revolutionizing the fields of biomedicine, bioimaging, and optoelectronics with their excellent physical, chemical, and biological properties. However, the difficulty of preparing excitation-dependent full-spectrum fluorescent CDs has seriously hindered their further research in fluorescence emission mechanisms and biomedicine. Here, we report full-spectrum fluorescent CDs that exhibit controlled emission changes from purple(380 nm) to red(613 nm) at room temperature by changing the excitation wavelength, and the excitation dependence was closely related to the regulation of sp2 and sp3 hybrid carbon structures by β-cyclodextrin-related groups. In addition,by regulating the content of β-cyclodextrin, the optimal quantum yields of full-spectrum fluorescent CDs were 8.97%, 8.35%, 7.90%, 9.69% and 17.4% at the excitation wavelengths of 340, 350, 390, 410 and 540 nm,respectively. Due to their excellent biocompatibility and color tunability, full-spectrum fluorescent CDs emitted bright and steady purple, blue, green, yellow, and red fluorescence in MCF-7 cells. Moreover, we optimized the imaging conditions of CDs and mitochondrial-specific dyes;and realized the mitochondrialtargeted co-localization imaging of purple, blue and green fluorescence. After that, we also explored the effect of full-spectrum fluorescent CDs in vivo fluorescence imaging through the intratumorally, subcutaneously, and caudal vein, and found that full-spectrum fluorescent CDs had good fluorescence imaging ability in vivo.

关 键 词：Carbon dots Multi-emission Fluorescence mechanism Mitochondrial targeting Color tenability Fluorescence imaging 

分 类 号：R313[医药卫生—基础医学] O657.3[理学—分析化学] TQ127.11[理学—化学]