深州蜜桃果实中的病毒和类病毒检测  

The Detection of Virus and Viroid in Shenzhoumi Peach Fruit

作　　者：叶茂 刘春生 刘亚婷 苏凯 张晨光 肖坤 肖啸[1,2] 张立彬 武军凯[1,2] YE Mao;LIU Chunsheng;LIU Yating;SU Kai;ZHANG Chenguang;XIAO Kun;XIAO Xiao;ZHANG Libin;WU Junkai(College of Horticultural Science&Technology,Hebei Normal University of Science&Technology,Qinhuangdao Hebei;Hebei Key Laboratory of Horticultural Germplasm Excavation and Innovative Utilization,China)

机构地区：[1]河北科技师范学院园艺科技学院,河北秦皇岛066600 [2]河北省特色园艺种质挖掘与创新利用重点实验室

出　　处：《河北科技师范学院学报》2023年第3期41-46,共6页Journal of Hebei Normal University of Science & Technology

基　　金：河北省现代农业产业技术体系桃创新团队项目(项目编号:HBCT2021220402);热杂果现代种业科技创新团队(项目编号:21326310D);承德市科技计划项目(项目编号:202201A066)。

摘　　要：为了确定侵染深州蜜桃的病毒和类病毒种类,利用RT-PCR和qRT-PCR的方法对侵染桃树的7种病毒和类病毒进行了检测。RT-PCR病毒鉴定结果表明,深州蜜桃感染了苹果褪绿叶斑病毒(ACLSV)、李树皮坏死茎纹孔伴随病毒(PBNSPaV)、李属坏死环斑病毒(PNRSV)和啤酒花矮化类病毒(HSVd),在深州蜜桃果实中没有鉴定到李矮缩病毒(PDV)、樱桃绿环斑驳病毒(CGRMV)、杏假褪绿叶斑病毒(APCLSV)。qRT-PCR检测结果进一步表明李属坏死环斑病毒(PNRSV)侵染了深州蜜桃,其病毒RNA表达量最低。利用RT-PCR开发出高效的多重检测体系,能够同时检测ACLSV,PBNSPaV和HSVd病毒。Seven viruses and viroid species infecting peach trees were examined with RT-PCR and qRT-PCR methods to investigate the virus and virus-like species infecting Shenzhoumi peach.The RT-PCR showed that Shenzhoumi peach was infected with apple chlorotic leaf spot virus(ACLSV),plum bark necrosis stem pitting-associated virus(PBNSPaV),plum necrotic ringspot virus(PNRSV)and hops stunt viroid(HSVd),but not with plum dwarf virus(PDV),cherry green ring mottle virus(CGRMV)or apricot pseudo-fading green leaf spot virus(APCLSV).The qRT-PCR showed that plum necrotic ringspot virus(PNRSV)infected Shenzhoumi peach with the lowest viral RNA expression.An efficient multiple detection system was developed with RT-PCR to detect ACLSV,PBNSPaV and HSVd,simultaneously.

关 键 词：深州蜜桃 病毒检测 RT-PCR QRT-PCR 

分 类 号：Q939.46[生物学—微生物学]