我国传统猪丹毒疫苗及流行株灭活疫苗的免疫保护效力评估  

Immune protective efficacy of China's traditional vaccines against the prevalent strains of Erysipelothrix rhusiopathiae

作　　者：詹鹏飞 裴世璇 宋吉健 韩忆侬 关丽君 薛云[1] 司丽芳[1,2] 赵战勤[1,2] ZHAN Peng-fei;PEI Shi-xuan;SONG Ji-jian;HAN Yi-nong;GUAN Li-jun;XUE Yun;SI Li-fang;ZHAO Zhan-qin(Laboratory of Veterinary biologics Engineering,College of Animal Science and Technology,Henan University of Science and Technology,Luoyang 471003,China;Key-Disciplines Lab of safety of environment and Animal Product,College of Animal Science and Technology,Henan University of Science and Technology,Luoyang 471003,China)

机构地区：[1]河南科技大学动物科技学院/兽医生物制品工程实验室,河南洛阳471003 [2]河南科技大学动物科技学院/河南省高等学校环境与畜产品安全重点学科开放实验室,河南洛阳471003

出　　处：《中国预防兽医学报》2023年第7期716-721,共6页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家自然科学基金项目(31672530、U1704117)。

摘　　要：为评估我国传统猪丹毒灭活疫苗(C43-5株,2型)及其衍生的猪丹毒活疫苗(GC42株和G4T10株)对当前流行株HG-1、Er-2、Er-3和Er-4(1a型)的免疫保护效力,本研究将2种猪丹毒活疫苗(GC42株和G4T10株)分别经皮下注射免疫小鼠,14 d后采用ELISA方法检测小鼠血清中的抗体水平,免疫后15 d,以10 LD50/只分别经腹腔注射攻击传统疫苗检验株C43-6、C43-8,当前流行株HG-1、Er-2、Er-3和Er-4菌液并观察、记录各组小鼠的临床症状和死亡情况,共观察14 d。死亡小鼠立即剖检,采集心、肺、肝等组织进行细菌的分离与鉴定。结果显示,2种活疫苗均能刺激小鼠产生较高的抗体水平,且极显著高于PBS对照组(P<0.01),但两种活疫苗之间无显著差异(P>0.05);攻毒结果显示,分别对应GC42株和G4T10株的6个攻毒组小鼠均无任何临床症状,且均获得100%的保护率;对照组小鼠攻毒后24 h出现相应发病症状,5 d内全部死亡;死亡小鼠各组织器官主要出现败血症的剖检病变;从死亡小鼠的心血、肺、肝等脏器均能分离到猪丹毒丝菌。将我国传统猪丹毒灭活疫苗株C43-5及其检验株C43-6和C43-8,以及当前流行株HG-1、Er-2、Er-3和Er-4分别制备灭活疫苗免疫小鼠2次(间隔21 d),于首免后20 d和35 d分别采用ELISA方法检测抗体水平;小鼠首免后35 d,按上述方法攻毒,并检测相应指标。结果显示,上述7种灭活疫苗免疫小鼠20 d后,均诱导小鼠产生了极显著高于对照组的Ig G抗体水平(P<0.01),但7种灭活疫苗之间均无显著差异(P>0.05);首免后35 d与首免后20 d相比,7种灭活疫苗免疫组小鼠的Ig G抗体水平虽有所升高,但均无显著差异(P>0.05)。攻毒结果显示,C43-5、C43-6、C43-8和HG-1株灭活疫苗免疫组小鼠均获得80%(4/5)及以上的保护率,Er-2、Er-3和Er-4株的灭活疫苗仅能对小鼠提供20%~60%(1/5~3/5)的保护率,6个菌株攻击的对照组小鼠均于攻毒后24 h开始发病,5 d内全部死亡。本研究To evaluate the immune protective efficacy of Chinese traditional swine erysipelas inactivated vaccine(C43-5strain,type 2) and its derived live vaccines( GC42 and G4T10),against the current prevalent Erysipelothrix rhusiopathiae strains HG-1,Er-2,Er-3 and Er-4(type 1a).In this study,two live swine erysipelas vaccines(GC42 strain and G4T10 strain) were injected subcutaneously immunized mice and 14 days later,the serum antibody levels of the mice were detected by ELISA method.Fifteen days after immunization,the conventional vaccine test strains C43-6 and C43-8,as well as the currently prevalent strains HG-1,Er-2,Er-3 and Er-4,were challenged intraperitoneally to immunized mice at a dose of 10LD50,respectively.The clinical symptoms and death of mice in each group were observed for 14 days.The dead mice were immediately dissected,and the heart,lung,liver and other tissues were collected for bacteria isolation and identification.The results showed that both kinds of live vaccines could stimulate mice to produce higher antibody level,which was significantly higher than that of PBS control group(P0.01),but there was no significant difference between the two kinds of vaccines(P0.05).The challenge results showed that the 6 challenge groups of mice corresponding to GC42 strain and G4T10 strain had no clinical symptoms,and all obtained 100%protection rate.The mice in the control group began to show corresponding symptoms 24 hours after the challenge,and all died within 5 days.The autopsy of the dead mice mainly showed septic pathological changes characterized by bleeding of various tissues and organs.E.rhusiopathiae can be isolated from the heart,blood,lungs,liver and other organ tissues of dead mice.The inactivated Chinese traditional swine erysipelas vaccine strain C43-5,its test strains C43-6 and C43-8,and the current epidemic strains HG-1,Er-2,Er-3 and Er-4 were prepared and immunized mice twice(at an interval of 21 days),and the antibody levels were detected by ELISA at 20 and 35 days after the first immunization,respe

关 键 词：猪丹毒丝菌 传统疫苗 小鼠 保护效力 

分 类 号：S852.65[农业科学—基础兽医学]