寒冷地区城镇污水处理厂氮转化功能菌群和功能基因季节变化特征分析  

Analysis of seasonal variation characteristics of nitrogen transformation functional bacteria and functional genes in urban sewage treatment plants in cold regions

作　　者：柳蒙蒙 陈亚松 魏源送[3] 齐嵘[3] 钟慧[3] 柴玉峰 陈彦霖 陈梅雪[3] LIU Mengmeng;CHEN Yasong;WEI Yuansong;QI Rong;ZHONG Hui;CHAI Yufeng;CHEN Yanlin;CHEN Meixue(Yangtze Eco-Environment Engineering Research Center,China Three Gorges Corporation,Beijing 100038,China;National Engineering Research Center for Ecological Environment of Yangtze River Economic Belt,Beijing 100038,China;Department of Water Pollution Control,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China)

机构地区：[1]中国长江三峡集团有限公司长江生态环境工程研究中心,北京100038 [2]长江经济带生态环境国家工程研究中心,北京100038 [3]中国科学院生态环境研究中心水污染控制实验室,北京100085

出　　处：《给水排水》2023年第10期38-43,52,共7页Water & Wastewater Engineering

基　　金：中国长江三峡集团有限公司自主科研项目(WWKY-2020-0519,WWKY-2021-0477);国家自然科学基金项目(52170062)

摘　　要：以华北寒冷地区某典型城镇污水处理厂CASS工艺为研究对象,对污水处理厂不同季节活性污泥样品进行微生物分析,考察不同季节活性污泥的群落结构变化,探究脱氮效果与氮转化功能基因赋存的相关性.结果显示,秋冬季节,Bacteroidetes相对丰度上升至31.45%和30.47%,冬季CASS池内Methylotenera(12.94%)、Zoogloea(8.74%)、Dechloromonas(2.15%)、Arcobacter(7.43%)、Thauera(2.11%)、Pseudomonas(0.43%)共占已知微生物菌属的33.80%.由微生物结果可以看出,春夏季节过渡到秋季稳定运行,冬季时期系统内反硝化细菌均有大幅增加.本研究对生物脱氮系统中的菌群结构和多样性做了系统分析,可为优化寒冷地区城镇污水处理厂脱氮效果提供理论基础.Taking the CASS process of a typical urban sewage treatment plant in the cold region of North China as an example,the microbial analysis of the activated sludge samples was carried out to investigate the community structure changes of activated sludge in different seasons,and to explore the correlation between nitrogen removal efficiency and nitrogen transfer function genes.The results showed that the relative abundance of Bacteroidetes increased to 31.45%and 30.47%in autumn and winter.In winter,Methylotenera(12.94%),Zoogloea(8.74%),Dechloromonas(2.15%),Arcobacter(7.43%),Thauera(2.11%)and Pseudomonas(0.43%)occupied the CASS pool 33.80%of the known microbial genera.It can be seen from the microbial results that the system operated stably from spring and summer to autumn,and the denitrifying bacteria increased significantly in winter.This study systematically analyzed the structure and diversity of microbial community in the biological nitrogen removal system,which can provide a theoretical basis for optimizing the nitrogen removal effect of urban sewage plants in cold areas.

关 键 词：CASS工艺 高通量测序 微生物菌群 氮转化功能基因 脱氮 

分 类 号：X703[环境科学与工程—环境工程] TU992[建筑科学—市政工程]