斑玉蕈重组异核体菌株节孢子遗传转化效率差异性分析  

Analyses of genetic transformation efficiency of arthroconidia in recombinant heterokaryon strains of Hypsizygus marmoreus

作　　者：姜珊 周陈力 尚俊军 李燕[2] 邹根 汪滢[2] 王刚[3] 鲍大鹏[1,2,4] JIANG Shan;ZHOU Chenli;SHANG Junjun;LI Yan;ZOU Gen;WANG Ying;WANG Gang;BAO Dapeng(College of Food Science,Shanghai Ocean University,Shanghai 201306,China;Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China;College of Wetland Science,Yancheng Teachers University,Yancheng 224000,Jiangsu,China;Shanxi Key Laboratory of Edible Fungi for Loess Plateau,Shanxi Agricultural University,Jinzhong 030801,Shanxi,China)

机构地区：[1]上海海洋大学食品学院,上海201306 [2]上海市农业科学院食用菌研究所,上海201403 [3]盐城师范学院湿地学院,江苏盐城224000 [4]山西农业大学黄土高原食用菌山西省重点实验室,山西晋中030801

出　　处：《菌物学报》2023年第10期2091-2099,共9页Mycosystema

基　　金：黄土高原食用菌山西省重点实验室开放课题。

摘　　要：在斑玉蕈Hypsizygusmarmoreus双核体菌株原生质体单核化过程中会存在大量出现的单核体和极少出现的单核体。为方便研究,将大量出现的单核体定义为强势核,极少出现的单核体定义为弱势核。为了研究这些不同类型的细胞核对斑玉蕈节孢子遗传转化效率的影响,将本研究室前期原生质体单核化工作中分离的10株强势核单核体和弱势核单核体菌株分别进行两两配对杂交,获得5株强势核重组异核体构成供试菌株Q组,以及5株弱势核重组异核体构成供试菌株R组,利用这些新的重组异核体菌株展开节孢子遗传转化实验。实验统计结果显示,Q组中5株强势核重组异核体Q1A、Q1B、Q2A、Q3A和Q3B的转化效率分别为81%、59%、57%、56%和53%,R组中的5株弱势核重组异核体R1A、R2A、R2B、R3B和R4B的转化效率分别为45%、45%、44%、46%和41%,Q组5株强势核重组异核体的转化效率均高于R组5株弱势核重组异核体,且Q组菌株的平均转化效率为61%,显著高于R组的平均转化效率44%。这种单核菌株间遗传转化效率存在明显差异的机制还需要进一步研究。There are a large number of dominant nucleus monokaryons and a few recessive nucleus monokaryons in the process of protoplast monokaryotization of Hypsizygus marmoreus.In order to study the effect of these different types of nuclei on the genetic transformation efficiency of arthroconidia,ten strains of dominant and recessive nuclear monokaryons isolated previously from protoplast monokaryotization were hybridized in pairs respectively.The test strain Q group consisted of five recombinant heterokaryons crossed by the strains with dominant nuclei,the test strain R group consisted of five recombinant heterokaryons crossed by the strains with recessive nuclei were obtained.The arthroconidia of these new recombinant heterokaryons were used to carry out the genetic transformation experiment.The experimental statistical results showed that the transformation efficiency of five recombinant heteronuclear strains Q1A,Q1B,Q2A,Q3A and Q3B in group Q were 81%,59%,57%,56%and 53%,respectively.The transformation efficiency of R1A,R2A,R2B,R3B and R4B in group R were 45%,45%,44%,46%and 41%,respectively.The transformation efficiency of the five dominant recombinant heterokaryons was higher than that of the five recessive recombinant heterokaryons.The average transformation efficiency of group Q was 61%,and significantly higher than that of group R(44%).The mechanism of the significant difference in genetic transformation efficiency between monokaryons needs further research.

关 键 词：斑玉蕈 转化效率 重组异核体 强势核 弱势核 

分 类 号：S646[农业科学—蔬菜学]