植物碳氮源浸提液对中国皱木耳“黔皱耳1号”液体培养及漆酶活性的影响  

Effects of plant carbon and nitrogen source extracts on liquid culture and laccase activities of Auricularia sinodelicata“Qian No.1”

作　　者：许娟 徐彦军[1] 邹琴[1] 查兴雪 XU Juan;XU Yanjun;ZOU Qin;ZHA Xingxue(Laboratory of Wild Mushroom Protection and Domestication Function,Guizhou Province Edible Mushroom Industry System,College of Agriculture,Guizhou University/Institute of Edible Mushroom Research,Guizhou University,Guiyang 550025,Guizhou,China)

机构地区：[1]贵州大学农学院/贵州大学食用菌研究院、贵州省食用菌产业体系野生菌保护抚育及驯化功能实验室,贵州贵阳550025

出　　处：《菌物学报》2023年第10期2119-2128,共10页Mycosystema

基　　金：贵州省科技计划项目(黔科合支撑[2022]重点025-8)。

摘　　要：为获得适宜的野生中国皱木耳液体发酵培养基,在常规液体PDA培养基中添加一定量的植物碳氮源浸提液,通过对不同碳氮源及其添加量和正交试验筛选,结果表明培养基中同时添加植物碳源小麦5g/L和氮源麦麸10g/L的配方组合培养6d时,其菌丝球生物量干重最高[(1.1580±0.1566)g/100 mL],菌丝球密度高,大小均一;比CK[(0.5948±0.0547)g/100 mL]提高了94.69%。各配方漆酶活性9 d内的酶活大小及变化与CK相比有显著不同,植物碳氮源添加物对漆酶的产生有一定的诱导效果,不同诱导培养基下发酵产漆酶活性除玉米粉和CK基质出现先升后降的趋势外,其余均随培养时间的增加,总体呈低幅度的下降趋势。本研究将为中国皱木耳的人工栽培液体菌种生产提供一定的理论基础。In order to obtain a suitable liquid fermentation medium for cultivation of wild Auricularia sinodelicata,a certain amount of plant carbon and nitrogen source leachate was added to the conventional liquid PDA medium,and the additional quantities of the leachate were optimized by orthogonal tests.It was showed that the highest mycelial ball biomass dry weight[(1.1580±0.1566)g/100 mL]was obtained in the culture incubated for 6 d under the addition of wheat grain 5 g/L and wheat bran 10 g/L.The mycelium balls showed high density and uniform size,and the mass dry weight was 94.69%higher than that of CK[(0.5948±0.0547)g/100 mL].The degree and change of laccase activities under different medium formulation within 9 d incubation were significantly different as compared with those of CK.The plant carbon and nitrogen source additive had a certain induction effect on the production of laccase,and the laccase activities under different induction media showed a trend of decrease to a certain extent with the increase of incubation time,except for medium with additional corn flour and CK,in which the laccase activities increase first and then decrease.This study provides reference for the production of artificially cultivated liquid strains of A.sinodelicata.

关 键 词：中国皱木耳 浸提液 菌丝生物量 漆酶活性 

分 类 号：S646.6[农业科学—蔬菜学]