球孢白僵菌与摩西球囊霉菌对烟草生长及品质的协同增效作用  

Synergistic effects of Beauveria bassiana and Glomus mosseae on growth and quality of tobacco

作　　者：高英明 刘京 成瑜 许忠顺 管景强 陈文敏 高贵 邹晓 GAO Yingming;LIU Jing;CHENG Yu;XU Zhongshun;GUAN Jingqiang;CHEN Wenmin;GAO Gui;ZOU Xiao(Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education),Collaborative Innovation Center for Mountain Ecology&Agro-bioengineering(CICMEAB),College of Life Sciences/Institute of Agro-bioengineering,Guizhou University,Guiyang 550025,Guizhou,China;Institute of Fungus Resources,Guizhou University,Guiyang 550025,Guizhou,China;Guizhou Tobacco Company Zunyi Branch,Zunyi 563000,Guizhou,China;Guizhou Tobacco Company Qianxinan Branch,Xingyi 562400,Guizhou,China)

机构地区：[1]贵州大学生命科学学院/农业生物工程研究院,山地植物资源保护与保护种质创新教育部重点实验室山地生态与农业生物工程协同创新中心,贵州贵阳550025 [2]贵州大学真菌资源研究所,贵州贵阳550025 [3]贵州省烟草公司遵义市公司,贵州遵义563000 [4]贵州省烟草公司黔西南州公司,贵州兴义562400

出　　处：《菌物学报》2023年第10期2129-2140,共12页Mycosystema

基　　金：中国烟草总公司贵州省公司重点研发项目(2022XM18);贵州省烟草公司遵义市公司技术开发(201802);贵州省烟草公司黔西南州公司技术开发(202204)。

摘　　要：本研究将球孢白僵菌Beauveria bassiana和摩西球囊霉菌Glomus mosseae作为混合菌剂接种于烟草。设置了未施菌(CK)、单施球孢白僵菌(BB)、单施摩西球囊霉菌(GM)和混合施菌(MIX)4个不同处理,并对田间烟草整个生长周期的农艺性状、叶绿素SPAD值、干物质积累和石油醚提取物(PEE)含量进行了测定。结果表明:混合施菌处理能显著提高苗期根系的农艺性状,表现出对根系的促生作用;幼苗的干物质积累也得到显著提升。移栽后,混合施菌处理的植株的最大叶面积、叶片数、茎围和株高相较于未施菌组均得到显著提升,植株叶绿素含量得到显著增加,干物质积累量提高了1.24-1.32倍。在移栽60 d后,MIX植株叶绿素含量显著降低,降幅达20.1%。混合施菌处理显著提高了上部成熟烟叶的PEE含量,其中在B2F和B3F等级烟叶中分别提高了5.70%和6.68%。球孢白僵菌和摩西球囊霉的混合应用在烟草不同生长阶段均具有协同增效作用,显著改善了烟草的根系发育,提高了植株的生长势和上部叶PEE的含量。In this study,Beauveria bassiana(BB)and Glomus mosseae(GM)as compound inoculants(MIX)were inoculated into tobacco.Four different treatments including CK,inoculation of sole BB,inoculation of sole GM and inoculation of MIX were set up.The agronomic characters,chlorophyll SPAD,dry matter accumulation and petroleum ether extract(PEE)content of tobacco in the whole growth cycle were determined.The results showed that the MIX treatment could significantly improve the agronomic characteristics and promote the growth of the root system at seedling stage,and the dry matter accumulation of the seedling was also significantly improved.After transplantation,the maximum leaf area,leaf number,stem circumference,and plant height of the tobacco plants treated with MIX were significantly increased as compared with those treated with other treatments.The chlorophyll content of plants was significantly increased,and the dry matter accumulation increased by 1.24−1.32 times.In 60 days after transplantation,the chlorophyll content of MIX-treated plants significantly decreased by 20.1%,and the PEE content was significantly increased by 5.70%and 6.68%in B2F and B3F grade of tobacco upper mature leaves,respectively.The results come to the conclusion that the combination of B.bassiana and G.mosseae has a synergistic effect in different growth stages of tobacco,significantly improving the root development of tobacco,increasing the plant growth potential and the content of PEE in upper leaves.

关 键 词：球孢白僵菌 丛植菌根真菌 摩西球囊霉菌 根系特征 协同增效 农艺性状 石油醚提取物 

分 类 号：S572[农业科学—烟草工业]